Experiment set12S323 for Pantoea sp. MT58

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Ammonium chloride nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + Ammonium chloride (100 mM)
Culturing: MT058_ML2
By: Alex Rivier on 1/9/25
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 10 genes in this experiment

For nitrogen source Ammonium chloride in Pantoea sp. MT58

For nitrogen source Ammonium chloride across organisms

SEED Subsystems

Subsystem #Specific
Biotin biosynthesis 2
Coenzyme A Biosynthesis 2
Thiamin biosynthesis 2
TCA Cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 3
phosphopantothenate biosynthesis I 4 4 2
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I 2 2 1
thiamine diphosphate biosynthesis I (E. coli) 2 2 1
thiamine diphosphate biosynthesis II (Bacillus) 2 2 1
biotin biosynthesis from 8-amino-7-oxononanoate II 4 3 2
biotin biosynthesis from 8-amino-7-oxononanoate III 5 3 2
thiamine diphosphate salvage V 3 3 1
biotin biosynthesis II 6 4 2
thiamine diphosphate biosynthesis III (Staphylococcus) 3 1 1
thiamine diphosphate biosynthesis IV (eukaryotes) 3 1 1
superpathway of thiamine diphosphate biosynthesis I 10 8 3
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) 7 3 2
superpathway of thiamine diphosphate biosynthesis II 11 9 3
phosphopantothenate biosynthesis III (archaea) 4 2 1
superpathway of coenzyme A biosynthesis I (bacteria) 9 9 2
thiamine diphosphate salvage II 5 5 1
biotin biosynthesis I 15 14 3
superpathway of coenzyme A biosynthesis II (plants) 10 5 2
thiazole component of thiamine diphosphate biosynthesis I 6 4 1
thiazole component of thiamine diphosphate biosynthesis II 7 5 1
thiamine diphosphate salvage IV (yeast) 7 5 1
partial TCA cycle (obligate autotrophs) 8 7 1
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) 8 4 1
TCA cycle VII (acetate-producers) 9 6 1
TCA cycle VI (Helicobacter) 9 6 1
TCA cycle I (prokaryotic) 10 9 1
superpathway of glyoxylate bypass and TCA 12 11 1
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass 26 25 1