Experiment set12IT092 for Desulfovibrio vulgaris Hildenborough JW710
MoLS4 without Pantothenic acid with Casamino acids (vit. assay) 0.05%
Group: nutrientMedia: MoLS4_no_Pantothenic_acid + Casamino acids (vitamin assay) (0.5 mg/mL), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 6/14/2017
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 20 mM Ammonium chloride, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix minus calcium pantothenate (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Aromatic amino acid degradation | 1 |
| Auxin biosynthesis | 1 |
| Coenzyme A Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Histidine metabolism
- Tyrosine metabolism
- Phenylalanine metabolism
- Tryptophan metabolism
- beta-Alanine metabolism
- Pantothenate and CoA biosynthesis
- Indole and ipecac alkaloid biosynthesis
- Alkaloid biosynthesis I
- Biosynthesis of alkaloids derived from shikimate pathway
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: