Experiment set12IT088 for Pseudomonas fluorescens FW300-N2E2
No vitamins, timepoint 2
Group: vitaminMedia: RCH2_defined_glucose_noVitamin
Culturing: pseudo6_N2E2_ML5b, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 12.4 generations
By: Adam on 21-Jun-16
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate)
Specific Phenotypes
For 4 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Bacterial Chemotaxis | 1 |
| Nudix proteins (nucleoside triphosphate hydrolases) | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I | 2 | 2 | 1 |
| superpathway of thiamine diphosphate biosynthesis III (eukaryotes) | 7 | 3 | 1 |
| superpathway of thiamine diphosphate biosynthesis I | 10 | 7 | 1 |
| superpathway of thiamine diphosphate biosynthesis II | 11 | 8 | 1 |