Experiment set12IT079 for Pseudomonas fluorescens SBW25-INTG

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Sucrose (C) and L-Glutamine (N); with MOPS; with PEG

Group: stress
Media: MME_noNitrogen_noCarbon + Sucrose (10 mM) + L-Glutamine (5 mM) + 3-(N-morpholino)propanesulfonic acid (20 mM) + PEG 6000 (25 vol%), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 33 (C), shaken=1300 rpm
By: Rob Egbert on 11-May-21
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 7 genes in this experiment

For stress Sucrose in Pseudomonas fluorescens SBW25-INTG

For stress Sucrose across organisms

SEED Subsystems

Subsystem #Specific
Chorismate Synthesis 1
Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 1
Nitric oxide synthase 1
Oxidative stress 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
superoxide radicals degradation 2 2 1
3-dehydroquinate biosynthesis I 2 2 1
reactive oxygen species degradation 4 4 1
chorismate biosynthesis I 7 7 1
ethene biosynthesis III (microbes) 7 6 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of L-tryptophan biosynthesis 13 13 1
superpathway of aromatic amino acid biosynthesis 18 18 1
superpathway of chorismate metabolism 59 42 1