Experiment set12IT077 for Pseudomonas fluorescens SBW25-INTG

Sucrose (C) and L-Glutamine (N); with MOPS; with PEG

Group: stress
Media: MME_noNitrogen_noCarbon + Sucrose (10 mM) + L-Glutamine (5 mM) + 3-(N-morpholino)propanesulfonic acid (20 mM) + PEG 6000 (25 vol%), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 33 (C), shaken=1300 rpm
By: Rob Egbert on 11-May-21
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 7 genes in this experiment

For stress Sucrose in Pseudomonas fluorescens SBW25-INTG

For stress Sucrose across organisms

SEED Subsystems

Subsystem	#Specific
Nitric oxide synthase	1
Oxidative stress	1
YeiH	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superoxide radicals degradation	2	2	1
reactive oxygen species degradation	4	4	1
ethene biosynthesis III (microbes)	7	6	1