Experiment set12IT070 for Ralstonia sp. UNC404CL21Col

Ra1_0.1

Group: phage
Media: LB + Kanamycin (0.05 mg/mL) + CaCl2, MgSO4 (5mM ), pH=7
Culturing: CL21_ML4, 96-well_plate, Aerobic, at 30 (C), shaken=567 rpm
By: Madeline on 1/13/2021
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride

Specific Phenotypes

For 22 genes in this experiment

For phage Kanamycin in Ralstonia sp. UNC404CL21Col

For phage Kanamycin across organisms

SEED Subsystems

Subsystem	#Specific
Oxidative stress	2
ATP-dependent RNA helicases, bacterial	1
Bacterial Chemotaxis	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
Cobalt-zinc-cadmium resistance	1
DNA Repair Base Excision	1
Flagellar motility	1
Phosphorylcholine incorporation in LPS	1
Ton and Tol transport systems	1
Two-component regulatory systems in Campylobacter	1
Type IV pilus	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine betaine biosynthesis I (Gram-negative bacteria)	2	2	1
glycine betaine biosynthesis II (Gram-positive bacteria)	2	2	1
choline degradation I	2	2	1
choline-O-sulfate degradation	3	3	1