Experiment set12IT059 for Paraburkholderia graminis OAS925

Compare to:

RCH2_defined_glucose_5mM with Adenine 2 mM

Group: stress
Media: RCH2_defined_glucose_5mM + Adenine hydrochloride hydrate (2 mM) + Potassium hydroxide (1 mM)
Culturing: Burkholderia_OAS925_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Albina on 15-Mar-22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 5 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 19 genes in this experiment

For stress Adenine hydrochloride hydrate in Paraburkholderia graminis OAS925

For stress Adenine hydrochloride hydrate across organisms

SEED Subsystems

Subsystem #Specific
Allantoin Utilization 2
Molybdenum cofactor biosynthesis 2
Photorespiration (oxidative C2 cycle) 2
Purine Utilization 2
Transport of Molybdenum 2
Carboxysome 1
D-galactarate, D-glucarate and D-glycerate catabolism 1
Lysine Biosynthesis DAP Pathway 1
Pyruvate metabolism I: anaplerotic reactions, PEP 1
Respiratory dehydrogenases 1 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-malate degradation I 1 1 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
glycolate and glyoxylate degradation I 4 3 2
NADH to cytochrome bd oxidase electron transfer I 2 1 1
adenosine nucleotides degradation II 5 4 2
molybdenum cofactor biosynthesis 3 3 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
superpathway of glycol metabolism and degradation 7 5 2
C4 photosynthetic carbon assimilation cycle, NADP-ME type 7 4 2
guanosine nucleotides degradation II 4 4 1
adenosine nucleotides degradation I 8 7 2
guanosine nucleotides degradation III 4 3 1
inosine 5'-phosphate degradation 4 3 1
D-glucarate degradation I 4 3 1
guanosine nucleotides degradation I 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
D-galactarate degradation I 4 3 1
superpathway of D-glucarate and D-galactarate degradation 5 4 1
purine nucleotides degradation II (aerobic) 11 8 2
purine nucleotides degradation I (plants) 12 10 2
methylgallate degradation 6 5 1
superpathway of guanosine nucleotides degradation (plants) 6 5 1
purine nucleobases degradation II (anaerobic) 24 16 4
NAD(P)/NADPH interconversion 6 3 1
Fe(II) oxidation 6 2 1
ureide biosynthesis 7 5 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 8 2
caffeine degradation III (bacteria, via demethylation) 7 1 1
protocatechuate degradation I (meta-cleavage pathway) 8 6 1
L-lysine biosynthesis I 9 9 1
superpathway of purines degradation in plants 18 14 2
theophylline degradation 9 1 1
superpathway of vanillin and vanillate degradation 10 6 1
caffeine degradation IV (bacteria, via demethylation and oxidation) 10 1 1
syringate degradation 12 7 1
gluconeogenesis I 13 11 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 15 1
aspartate superpathway 25 22 1
superpathway of microbial D-galacturonate and D-glucuronate degradation 31 19 1