Experiment set12IT039 for Paraburkholderia graminis OAS925
RCH2_defined_glucose_5mM with Pyridoxal hydrochloride 1 mM
Group: stressMedia: RCH2_defined_glucose_5mM + Pyridoxal hydrochloride (1 mM) + Dimethyl Sulfoxide (1 vol%)
Culturing: Burkholderia_OAS925_ML2, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Albina on 15-Mar-22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 5 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 12 genes in this experiment
For stress Pyridoxal hydrochloride in Paraburkholderia graminis OAS925
For stress Pyridoxal hydrochloride across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| 2-aminoethylphosphonate biosynthesis | 3 | 3 | 1 |
| methylphosphonate biosynthesis | 4 | 2 | 1 |
| peptidoglycan maturation (meso-diaminopimelate containing) | 12 | 4 | 2 |
| fosfomycin biosynthesis | 7 | 2 | 1 |
| FR-900098 and FR-33289 antibiotics biosynthesis | 10 | 1 | 1 |
| rhizocticin A and B biosynthesis | 12 | 2 | 1 |
| dehydrophos biosynthesis | 13 | 2 | 1 |
| peptidoglycan biosynthesis IV (Enterococcus faecium) | 17 | 12 | 1 |
| peptidoglycan biosynthesis II (staphylococci) | 17 | 12 | 1 |
| phosalacine biosynthesis | 25 | 6 | 1 |
| phosphinothricin tripeptide biosynthesis | 25 | 6 | 1 |