Experiment set12IT033 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with Deoxycholic acid 0.2 mg/ml

Group: stress
Media: Varel_Bryant_medium_Glucose + Deoxycholic acid (0.2 mg/ml) + Ethanol (0.4 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2050 C5

Specific Phenotypes

For 6 genes in this experiment

For stress Deoxycholic acid in Bacteroides thetaiotaomicron VPI-5482

For stress Deoxycholic acid across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 3

Metabolic Maps

Color code by fitness: see overview map or list of maps.