Experiment set12IT031 for Pseudomonas syringae pv. syringae B728a

KB with Novobiocin sodium salt 250 ug/ml

Group: stress
Media: KB + Novobiocin sodium salt (250 ug/ml)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/9/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate

Specific Phenotypes

For 15 genes in this experiment

For stress Novobiocin sodium salt in Pseudomonas syringae pv. syringae B728a

For stress Novobiocin sodium salt across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1)	1
DNA-replication	1
DNA repair, bacterial RecFOR pathway	1
Histidine Degradation	1
Murein hydrolase regulation and cell death	1
Ribosome biogenesis bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pyrimidine metabolism
• Purine metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pseudouridine degradation	2	1	1
Ac/N-end rule pathway	21	6	6
Arg/N-end rule pathway (eukaryotic)	14	8	1