Sodium malate (C) and Ammonium chloride (N); with MOPS
Group:
stress
Media:
MME_noNitrogen_noCarbon +
Sodium malate (30 mM) +
Ammonium chloride (10 mM) +
3-(N-morpholino)propanesulfonic acid (20 mM) +
Sodium Chloride (400 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 8 (C), shaken=1300 rpm
By: Rob Egbert on
11-May-21
Media components: 9.1 mM
Potassium phosphate dibasic trihydrate, 20 mM
3-(N-morpholino)propanesulfonic acid, 4.3 mM
Sodium Chloride, 0.41 mM
Magnesium Sulfate Heptahydrate, 0.07 mM
Calcium chloride dihydrate, MME Trace Minerals
(0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 6 genes in this experiment
For stress Sodium malate in Pseudomonas fluorescens SBW25-INTG
For stress Sodium malate across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|
| trehalose biosynthesis IV | 1 | 1 | 1 |
| 4-coumarate degradation (aerobic) | 5 | 3 | 2 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| 4-coumarate degradation (anaerobic) | 6 | 2 | 2 |
| starch degradation V | 4 | 3 | 1 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 3 |
| adipate degradation | 5 | 5 | 1 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 4 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 4 | 1 |
| adipate biosynthesis | 5 | 4 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 4 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| oleate β-oxidation | 35 | 33 | 6 |
| fatty acid salvage | 6 | 6 | 1 |
| L-isoleucine degradation I | 6 | 4 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 4 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 6 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 5 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| partial TCA cycle (obligate autotrophs) | 8 | 8 | 1 |
| glycogen degradation I | 8 | 6 | 1 |
| L-valine degradation I | 8 | 6 | 1 |
| sucrose biosynthesis II | 8 | 6 | 1 |
| pyruvate fermentation to butanol I | 8 | 4 | 1 |
| glycogen biosynthesis III (from α-maltose 1-phosphate) | 8 | 3 | 1 |
| TCA cycle VI (Helicobacter) | 9 | 7 | 1 |
| TCA cycle VII (acetate-producers) | 9 | 7 | 1 |
| valproate β-oxidation | 9 | 6 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 4 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| starch degradation II | 9 | 1 | 1 |
| TCA cycle I (prokaryotic) | 10 | 9 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 6 | 1 |
| 3-phenylpropanoate degradation | 10 | 5 | 1 |
| superpathway of phenylethylamine degradation | 11 | 6 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 2 |
| superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 3 | 1 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 5 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 3 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 3 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 6 | 1 |
| glycerol degradation to butanol | 16 | 10 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 4 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 7 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 4 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 8 | 1 |
| toluene degradation VI (anaerobic) | 18 | 4 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 22 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 1 |