Experiment set12IT030 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with Chenodeoxycholic acid 0.5 mg/ml

Group: stress
Media: Varel_Bryant_medium_Glucose + Chenodeoxycholic acid (0.5 mg/ml) + Ethanol (2.5 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2050 C2

Specific Phenotypes

For 13 genes in this experiment

For stress Chenodeoxycholic acid in Bacteroides thetaiotaomicron VPI-5482

For stress Chenodeoxycholic acid across organisms

SEED Subsystems

Subsystem #Specific
High affinity phosphate transporter and control of PHO regulon 1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1
Phosphate metabolism 1
Predicted carbohydrate hydrolases 1
Purine conversions 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
guanosine deoxyribonucleotides de novo biosynthesis I 2 2 1
CMP phosphorylation 2 2 1
adenosine deoxyribonucleotides de novo biosynthesis I 2 2 1
pyrimidine deoxyribonucleotide phosphorylation 4 3 2
superpathway of guanosine nucleotides de novo biosynthesis I 6 6 2
UTP and CTP de novo biosynthesis 3 3 1
pyrimidine deoxyribonucleotides de novo biosynthesis I 9 8 3
pyrimidine deoxyribonucleotides de novo biosynthesis III 9 7 3
purine deoxyribonucleosides salvage 6 4 2
pyrimidine deoxyribonucleotides de novo biosynthesis IV 7 5 2
superpathway of pyrimidine nucleobases salvage 4 4 1
guanosine ribonucleotides de novo biosynthesis 4 4 1
superpathway of guanosine nucleotides de novo biosynthesis II 8 7 2
pyrimidine deoxyribonucleotides biosynthesis from CTP 8 6 2
guanosine deoxyribonucleotides de novo biosynthesis II 4 3 1
adenosine deoxyribonucleotides de novo biosynthesis II 4 3 1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis 18 16 4
superpathway of pyrimidine deoxyribonucleoside salvage 9 7 2
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 10 3
superpathway of adenosine nucleotides de novo biosynthesis I 5 5 1
superpathway of pyrimidine ribonucleosides salvage 10 9 2
dZTP biosynthesis 5 4 1
ppGpp metabolism 6 6 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 3
superpathway of purine nucleotide salvage 14 13 2
superpathway of adenosine nucleotides de novo biosynthesis II 7 6 1
pyrimidine deoxyribonucleotides de novo biosynthesis II 7 3 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 3
superpathway of pyrimidine ribonucleotides de novo biosynthesis 9 8 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 4
plasmalogen biosynthesis I (aerobic) 16 2 1