Experiment set12IT030 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with Chenodeoxycholic acid 0.5 mg/ml

Group: stress
Media: Varel_Bryant_medium_Glucose + Chenodeoxycholic acid (0.5 mg/ml) + Ethanol (2.5 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2050 C2

Specific Phenotypes

For 12 genes in this experiment

For stress Chenodeoxycholic acid in Bacteroides thetaiotaomicron VPI-5482

For stress Chenodeoxycholic acid across organisms

SEED Subsystems

Subsystem #Specific
High affinity phosphate transporter and control of PHO regulon 1
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1
Phosphate metabolism 1
Predicted carbohydrate hydrolases 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
plasmalogen biosynthesis I (aerobic) 16 2 1