Experiment set12IT028 for Pseudomonas syringae pv. syringae B728a
KB with Fusaric acid 250 ug/ml
Group: stressMedia: KB + Fusaric acid (250 ug/ml)
Culturing: SyringaeB728a_ML2, 24 well microplate, Aerobic, at 28 (C), shaken=250 rpm
By: Tyler Helmann on 7/9/19
Media components: 10 g/L Bacto Peptone, 1.5 g/L Potassium phosphate dibasic, 15 g/L Glycerol, 0.6 g/L Magnesium sulfate
Specific Phenotypes
For 12 genes in this experiment
For stress Fusaric acid in Pseudomonas syringae pv. syringae B728a
For stress Fusaric acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Terminal cytochrome d ubiquinol oxidases | 2 |
| Terminal cytochrome oxidases | 2 |
| Alginate metabolism | 1 |
| Copper homeostasis | 1 |
| Lipid A modifications | 1 |
| Mannose Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Fructose and mannose metabolism
- Glycine, serine and threonine metabolism
- Benzoxazinone biosynthesis
- Nucleotide sugars metabolism
- Diterpenoid biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-mannose degradation I | 2 | 1 | 1 |
| D-mannose degradation II | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 3 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| Ac/N-end rule pathway | 21 | 6 | 6 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| Arg/N-end rule pathway (eukaryotic) | 14 | 8 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |