Experiment set12IT025 for Burkholderia phytofirmans PsJN

Disodium Glutarate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Disodium Glutarate (10 mM)
Culturing: BFirm_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 3.0 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 8 genes in this experiment

For carbon source Disodium Glutarate in Burkholderia phytofirmans PsJN

For carbon source Disodium Glutarate across organisms

SEED Subsystems

Subsystem	#Specific
Homogentisate pathway of aromatic compound degradation	2
Protocatechuate branch of beta-ketoadipate pathway	1
Xylose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Lysine degradation
• Tryptophan metabolism
• Benzoate degradation via CoA ligation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-lysine degradation IV	5	2	1
L-lysine degradation X	6	4	1
superpathway of L-lysine degradation	43	11	1