Experiment set12IT024 for Klebsiella michiganensis M5al

RCH2_defined_glucose with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.5 x; and 2,2'-Dipyridyl 50 uM

Group: supernatant
Media: RCH2_defined_glucose + supernatant from Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted (0.5 x) + 2,2'-Dipyridyl (50 uM)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 29-Jun-18
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Koxy-Set12-A D6

Specific Phenotypes

For 7 genes in this experiment

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem	#Specific
Lipid A-Ara4N pathway ( Polymyxin resistance )	1
Multidrug Resistance Efflux Pumps	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Puromycin biosynthesis
• Methionine metabolism
• Lysine biosynthesis
• Lysine degradation
• Arginine and proline metabolism
• Aminophosphonate metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Caprolactam degradation

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
polymyxin resistance	6	5	2