Experiment set12IT024 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant_medium_Glucose with Deoxycholic acid 0.2 mg/ml
Group: stressMedia: Varel_Bryant_medium_Glucose + Deoxycholic acid (0.2 mg/ml) + Ethanol (0.4 vol%)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2050 A5
Specific Phenotypes
For 6 genes in this experiment
For stress Deoxycholic acid in Bacteroides thetaiotaomicron VPI-5482
For stress Deoxycholic acid across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria | 3 |
| Respiratory Complex I | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 2 | 1 |
| NAD(P)/NADPH interconversion | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 1 | 1 |