Experiment set12IT009 for Bacteroides thetaiotaomicron VPI-5482

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Varel_Bryant_medium_Glucose with Bile salts 0.25 mg/mL; pH 7

Group: stress
Media: Varel_Bryant_medium_Glucose + Bile salts (0.25 mg/ml), pH=7
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: VB A E4

Specific Phenotypes

For 1 genes in this experiment

For stress Bile salts in Bacteroides thetaiotaomicron VPI-5482

For stress Bile salts across organisms

SEED Subsystems

Subsystem #Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.