Experiment set12IT002 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with Bile salts 0.5 mg/mL; pH 7

Group: stress
Media: Varel_Bryant_medium_Glucose + Bile salts (0.5 mg/ml), pH=7
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: VB B E3

Specific Phenotypes

For 9 genes in this experiment

For stress Bile salts in Bacteroides thetaiotaomicron VPI-5482

For stress Bile salts across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria	3
CMP-N-acetylneuraminate Biosynthesis	1
Maltose and Maltodextrin Utilization	1
Sialic Acid Metabolism	1
Trehalose Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Aminosugars metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
UDP-N-acetyl-α-D-mannosamine biosynthesis	1	1	1
CMP-N-acetylneuraminate biosynthesis II (bacteria)	3	2	1
UDP-N-acetyl-β-L-fucosamine biosynthesis	4	2	1
CMP-N-acetylneuraminate biosynthesis I (eukaryotes)	5	1	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	7	2
superpathway of CMP-sialic acids biosynthesis	15	6	1