Experiment set12IT001 for Bacteroides thetaiotaomicron VPI-5482
Varel_Bryant_medium_Glucose with Bile salts 0.5 mg/mL; pH 7
Group: stressMedia: Varel_Bryant_medium_Glucose + Bile salts (0.5 mg/ml), pH=7
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: VB A E3
Specific Phenotypes
For 13 genes in this experiment
For stress Bile salts in Bacteroides thetaiotaomicron VPI-5482
For stress Bile salts across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NADH to cytochrome bd oxidase electron transfer I | 2 | 1 | 1 |
| NADH to cytochrome bo oxidase electron transfer I | 2 | 1 | 1 |
| aerobic respiration III (alternative oxidase pathway) | 3 | 2 | 1 |
| aerobic respiration I (cytochrome c) | 4 | 2 | 1 |
| NAD(P)/NADPH interconversion | 6 | 2 | 1 |
| Fe(II) oxidation | 6 | 1 | 1 |