Experiment set12H9 for Pseudomonas stutzeri RCH2
L-Glutamine carbon source
Group: carbon sourceMedia: RCH2_defined_noCarbon + L-Glutamine (20 mM), pH=7.2
Culturing: psRCH2_ML7c, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 6.0 generations
By: Kelly on 2/25/2014
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 10 genes in this experiment
For carbon source L-Glutamine in Pseudomonas stutzeri RCH2
For carbon source L-Glutamine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis | 2 |
| Bacterial Chemotaxis | 1 |
| Flagellar motility | 1 |
| Glutamate dehydrogenases | 1 |
| Polyamine Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glutamate metabolism
- Arginine and proline metabolism
- Nitrogen metabolism
- D-Glutamine and D-glutamate metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-glutamate degradation I | 1 | 1 | 1 |
| L-glutamine degradation I | 1 | 1 | 1 |
| L-glutamate biosynthesis I | 2 | 2 | 1 |
| ammonia assimilation cycle III | 3 | 3 | 1 |
| L-alanine degradation II (to D-lactate) | 3 | 3 | 1 |
| ethene biosynthesis IV (engineered) | 3 | 1 | 1 |
| glutaminyl-tRNAgln biosynthesis via transamidation | 4 | 4 | 1 |
| L-asparagine biosynthesis III (tRNA-dependent) | 4 | 4 | 1 |
| L-glutamate and L-glutamine biosynthesis | 7 | 5 | 1 |
| L-glutamate degradation XI (reductive Stickland reaction) | 7 | 4 | 1 |
| 4-aminobutanoate degradation V | 7 | 3 | 1 |
| L-citrulline biosynthesis | 8 | 7 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 7 | 1 |
| superpathway of L-citrulline metabolism | 12 | 9 | 1 |
| methylaspartate cycle | 19 | 9 | 1 |