Experiment set11S524 for Enterobacter sp. TBS_079

0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010

Group: mixed community
Media: LB (0.1x) + 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010
Culturing: Enterobacter_TBS_079_ML3, agar plate, Aerobic, at 30 (C), (Solid)
By: on 3/19/24
Media components: 1 g/L Tryptone, 0.5 g/L Yeast Extract, 0.5 g/L Sodium Chloride (final concentrations)

Specific Phenotypes

For 14 genes in this experiment

For mixed community 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010 in Enterobacter sp. TBS_079

For mixed community 0.1X LB plate, 1:1 coculture with Pseudomonas silesiensis TBS_010 across organisms

SEED Subsystems

Subsystem	#Specific
Alanine biosynthesis	1
Glycogen metabolism	1
Queuosine-Archaeosine Biosynthesis	1
Ribonucleotide reduction	1
Staphylococcal phi-Mu50B-like prophages	1
Transport of Manganese	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycolysis / Gluconeogenesis
• Fatty acid metabolism
• Purine metabolism
• Pyrimidine metabolism
• Glycine, serine and threonine metabolism
• Tyrosine metabolism
• Selenoamino acid metabolism
• Starch and sucrose metabolism
• Nucleotide sugars metabolism
• Pyruvate metabolism
• 1- and 2-Methylnaphthalene degradation
• 3-Chloroacrylic acid degradation
• Butanoate metabolism
• Thiamine metabolism
• Retinol metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
ethanol degradation I	2	2	2
acetaldehyde biosynthesis I	1	1	1
L-cysteine degradation IV	1	1	1
L-alanine biosynthesis III	1	1	1
pyruvate fermentation to ethanol III	3	3	2
pyruvate fermentation to ethanol I	3	3	2
pyruvate fermentation to ethanol II	2	2	1
L-threonine degradation IV	2	2	1
cytidylyl molybdenum cofactor sulfurylation	2	1	1
ethanolamine utilization	5	4	2
acetylene degradation (anaerobic)	5	4	2
ethanol degradation II	3	3	1
2-deoxy-D-ribose degradation I	3	3	1
2-deoxy-α-D-ribose 1-phosphate degradation	3	3	1
L-leucine degradation III	3	2	1
L-methionine degradation III	3	2	1
L-isoleucine degradation II	3	2	1
L-valine degradation II	3	2	1
2-aminoethylphosphonate degradation I	3	1	1
2-hydroxypenta-2,4-dienoate degradation	3	1	1
sulfoacetaldehyde degradation IV	3	1	1
bis(guanylyl molybdopterin) cofactor sulfurylation	3	1	1
pyrimidine deoxyribonucleotides de novo biosynthesis II	7	7	2
thiazole component of thiamine diphosphate biosynthesis II	7	5	2
adenosine deoxyribonucleotides de novo biosynthesis II	4	4	1
glycogen biosynthesis I (from ADP-D-Glucose)	4	4	1
guanosine deoxyribonucleotides de novo biosynthesis II	4	4	1
superpathway of L-alanine biosynthesis	4	4	1
phytol degradation	4	3	1
L-phenylalanine degradation III	4	2	1
salidroside biosynthesis	4	2	1
L-tyrosine degradation III	4	2	1
tRNA-uridine 2-thiolation (mammalian mitochondria)	4	1	1
tRNA-uridine 2-thiolation (yeast mitochondria)	4	1	1
superpathway of fermentation (Chlamydomonas reinhardtii)	9	9	2
pyruvate fermentation to isobutanol (engineered)	5	4	1
(S)-propane-1,2-diol degradation	5	3	1
glucosylglycerol biosynthesis	5	3	1
[2Fe-2S] iron-sulfur cluster biosynthesis	10	4	2
phenylethanol biosynthesis	5	2	1
catechol degradation I (meta-cleavage pathway)	5	1	1
tRNA-uridine 2-thiolation (thermophilic bacteria)	5	1	1
superpathway of thiamine diphosphate biosynthesis II	11	9	2
molybdopterin biosynthesis	6	6	1
thiazole component of thiamine diphosphate biosynthesis I	6	6	1
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	1
triethylamine degradation	6	1	1
superpathway of Clostridium acetobutylicum solventogenic fermentation	13	6	2
noradrenaline and adrenaline degradation	13	4	2
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli)	14	14	2
superpathway of purine deoxyribonucleosides degradation	7	7	1
superpathway of adenosine nucleotides de novo biosynthesis II	7	7	1
3-methylbutanol biosynthesis (engineered)	7	6	1
serotonin degradation	7	3	1
catechol degradation II (meta-cleavage pathway)	7	1	1
toluene degradation I (aerobic) (via o-cresol)	7	1	1
toluene degradation V (aerobic) (via toluene-cis-diol)	7	1	1
mixed acid fermentation	16	16	2
superpathway of guanosine nucleotides de novo biosynthesis II	8	8	1
butanol and isobutanol biosynthesis (engineered)	8	3	1
3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation	8	2	1
p-cumate degradation	8	1	1
tRNA-uridine 2-thiolation (cytoplasmic)	8	1	1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation	17	8	2
heterolactic fermentation	18	15	2
hexitol fermentation to lactate, formate, ethanol and acetate	19	18	2
superpathway of thiamine diphosphate biosynthesis I	10	10	1
starch biosynthesis	10	6	1
meta cleavage pathway of aromatic compounds	10	1	1
superpathway of N-acetylneuraminate degradation	22	19	2
tRNA-uridine 2-thiolation and selenation (bacteria)	11	7	1
p-cymene degradation	11	1	1
naphthalene degradation to acetyl-CoA	12	2	1
L-tryptophan degradation IX	12	1	1
L-tryptophan degradation XII (Geobacillus)	12	1	1
superpathway of purine nucleotides de novo biosynthesis II	26	26	2
L-tryptophan degradation V (side chain pathway)	13	1	1
toluene degradation IV (aerobic) (via catechol)	13	1	1
superpathway of L-threonine metabolism	18	16	1
mandelate degradation to acetyl-CoA	18	3	1
superpathway of anaerobic sucrose degradation	19	17	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	2
superpathway of aerobic toluene degradation	30	4	1
superpathway of aromatic compound degradation via 3-oxoadipate	35	7	1
superpathway of aromatic compound degradation via 2-hydroxypentadienoate	42	6	1