Experiment set11S386 for Marinobacter adhaerens HP15
monoculture; Experiment A, time point 2
Group: pro99_seawaterMedia: Pro99_seawater
Culturing: Marino_ML2_MIT, bottle_100mL, Aerobic, at 24 (C), shaken=0 rpm
By: Christine Ziegler on 12/1/2024
Media components: 50 uM Sodium phosphate monobasic monohydrate, 800 uM Ammonium chloride, 1.17 uM EDTA (disodium salt), 1.18 uM Ferric chloride, 0.008 uM Zinc sulfate heptahydrate, 0.005 uM Cobalt chloride hexahydrate, 0.09 uM Manganese (II) chloride tetrahydrate, 0.003 uM Sodium Molybdate Dihydrate, 0.01 uM Sodium selenite pentahydrate, 0.01 uM Nickel (II) chloride hexahydrate, 1 X Filtered Seawater
Specific Phenotypes
For 1 genes in this experiment
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Branched-Chain Amino Acid Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Valine, leucine and isoleucine biosynthesis
- Pantothenate and CoA biosynthesis
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-valine biosynthesis | 4 | 4 | 1 |
| pyruvate fermentation to isobutanol (engineered) | 5 | 4 | 1 |
| L-isoleucine biosynthesis IV | 6 | 4 | 1 |
| L-isoleucine biosynthesis I (from threonine) | 7 | 7 | 1 |
| L-isoleucine biosynthesis III | 7 | 4 | 1 |
| L-isoleucine biosynthesis II | 8 | 5 | 1 |
| superpathway of branched chain amino acid biosynthesis | 17 | 17 | 2 |
| superpathway of L-isoleucine biosynthesis I | 13 | 13 | 1 |
| superpathway of L-threonine metabolism | 18 | 12 | 1 |