Experiment set11IT081 for Bacteroides thetaiotaomicron VPI-5482

palatinose hydrate carbon source

Group: carbon source
Media: Varel_Bryant_medium + palatinose hydrate (20 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2046 B02

Specific Phenotypes

For 2 genes in this experiment

For carbon source palatinose hydrate in Bacteroides thetaiotaomicron VPI-5482

For carbon source palatinose hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Inositol catabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Streptomycin biosynthesis
• Inositol phosphate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
myo-inositol degradation II	5	1	1
myo-inositol degradation I	7	1	1
myo-, chiro- and scyllo-inositol degradation	10	1	1
streptomycin biosynthesis	18	3	1