Experiment set11IT078 for Bacteroides thetaiotaomicron VPI-5482
Stachyose - 70% carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Stachyose - 70% (20 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2046 A02
Specific Phenotypes
For 1 genes in this experiment
For carbon source Stachyose - 70% in Bacteroides thetaiotaomicron VPI-5482
For carbon source Stachyose - 70% across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| TCA Cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Citrate cycle (TCA cycle)
- Glutathione metabolism
- Reductive carboxylate cycle (CO2 fixation)
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| cytosolic NADPH production (yeast) | 5 | 3 | 1 |
| nitrogen remobilization from senescing leaves | 8 | 5 | 1 |
| partial TCA cycle (obligate autotrophs) | 8 | 5 | 1 |
| superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) | 8 | 3 | 1 |
| TCA cycle V (2-oxoglutarate synthase) | 9 | 8 | 1 |
| TCA cycle VI (Helicobacter) | 9 | 6 | 1 |
| TCA cycle IV (2-oxoglutarate decarboxylase) | 9 | 6 | 1 |
| TCA cycle VII (acetate-producers) | 9 | 6 | 1 |
| TCA cycle I (prokaryotic) | 10 | 7 | 1 |
| reductive TCA cycle I | 11 | 9 | 1 |
| reductive TCA cycle II | 12 | 8 | 1 |
| superpathway of glyoxylate bypass and TCA | 12 | 7 | 1 |
| mixed acid fermentation | 16 | 12 | 1 |
| methylaspartate cycle | 19 | 11 | 1 |
| ethene biosynthesis V (engineered) | 25 | 15 | 1 |
| superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 18 | 1 |