Experiment set11IT073 for Bacteroides thetaiotaomicron VPI-5482
Laminaribiose carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Laminaribiose (20 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Adam on 6/29/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Growth plate: 2044 B03
Specific Phenotypes
For 3 genes in this experiment
For carbon source Laminaribiose in Bacteroides thetaiotaomicron VPI-5482
For carbon source Laminaribiose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Beta-Glucoside Metabolism | 1 |
| Iron acquisition in Vibrio | 1 |
| Ton and Tol transport systems | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| neolinustatin bioactivation | 3 | 2 | 2 |
| linustatin bioactivation | 4 | 2 | 2 |
| linamarin degradation | 2 | 1 | 1 |
| lotaustralin degradation | 2 | 1 | 1 |
| cellulose degradation II (fungi) | 3 | 2 | 1 |
| coumarin biosynthesis (via 2-coumarate) | 5 | 2 | 1 |
| α-tomatine degradation | 6 | 1 | 1 |
| firefly bioluminescence | 14 | 2 | 1 |