Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 33 (C), shaken=1300 rpm
| Pathway | #Steps | #Present | #Specific |
|---|
| trehalose biosynthesis IV | 1 | 1 | 1 |
| cis-cyclopropane fatty acid (CFA) biosynthesis | 1 | 1 | 1 |
| starch degradation V | 4 | 3 | 3 |
| pentose phosphate pathway (partial) | 3 | 3 | 2 |
| glycogen degradation I | 8 | 6 | 5 |
| β-alanine degradation II | 2 | 2 | 1 |
| putrescine degradation V | 2 | 2 | 1 |
| UDP-α-D-glucose biosynthesis | 2 | 2 | 1 |
| superoxide radicals degradation | 2 | 2 | 1 |
| glycogen degradation II | 6 | 5 | 3 |
| starch degradation III | 4 | 2 | 2 |
| sterculate biosynthesis | 2 | 1 | 1 |
| pentose phosphate pathway (non-oxidative branch) I | 5 | 5 | 2 |
| 4-coumarate degradation (aerobic) | 5 | 3 | 2 |
| sucrose biosynthesis II | 8 | 6 | 3 |
| benzoyl-CoA biosynthesis | 3 | 3 | 1 |
| pentose phosphate pathway (non-oxidative branch) II | 6 | 5 | 2 |
| D-apiose degradation I | 3 | 2 | 1 |
| GDP-α-D-glucose biosynthesis | 3 | 2 | 1 |
| sucrose degradation I (sucrose phosphotransferase) | 3 | 2 | 1 |
| trehalose degradation V | 3 | 2 | 1 |
| 4-coumarate degradation (anaerobic) | 6 | 2 | 2 |
| pentose phosphate pathway | 8 | 8 | 2 |
| reactive oxygen species degradation | 4 | 4 | 1 |
| glycogen biosynthesis I (from ADP-D-Glucose) | 4 | 3 | 1 |
| sucrose degradation IV (sucrose phosphorylase) | 4 | 3 | 1 |
| glycogen biosynthesis III (from α-maltose 1-phosphate) | 8 | 3 | 2 |
| formaldehyde assimilation II (assimilatory RuMP Cycle) | 9 | 6 | 2 |
| 2-methyl-branched fatty acid β-oxidation | 14 | 11 | 3 |
| L-arginine degradation II (AST pathway) | 5 | 5 | 1 |
| dTDP-β-L-rhamnose biosynthesis | 5 | 5 | 1 |
| adipate degradation | 5 | 5 | 1 |
| Rubisco shunt | 10 | 8 | 2 |
| adipate biosynthesis | 5 | 4 | 1 |
| fatty acid β-oxidation II (plant peroxisome) | 5 | 4 | 1 |
| glucose and glucose-1-phosphate degradation | 5 | 4 | 1 |
| sucrose degradation II (sucrose synthase) | 5 | 4 | 1 |
| fatty acid β-oxidation IV (unsaturated, even number) | 5 | 4 | 1 |
| 4-hydroxybenzoate biosynthesis III (plants) | 5 | 4 | 1 |
| glutaryl-CoA degradation | 5 | 3 | 1 |
| D-galactose degradation I (Leloir pathway) | 5 | 3 | 1 |
| glucosylglycerol biosynthesis | 5 | 2 | 1 |
| benzoate biosynthesis III (CoA-dependent, non-β-oxidative) | 5 | 2 | 1 |
| CDP-6-deoxy-D-gulose biosynthesis | 5 | 1 | 1 |
| pyruvate fermentation to hexanol (engineered) | 11 | 7 | 2 |
| (8E,10E)-dodeca-8,10-dienol biosynthesis | 11 | 6 | 2 |
| oleate β-oxidation | 35 | 33 | 6 |
| fatty acid salvage | 6 | 6 | 1 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | 12 | 10 | 2 |
| β-alanine biosynthesis II | 6 | 5 | 1 |
| methyl ketone biosynthesis (engineered) | 6 | 4 | 1 |
| pyruvate fermentation to butanol II (engineered) | 6 | 4 | 1 |
| superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis | 6 | 4 | 1 |
| L-isoleucine degradation I | 6 | 4 | 1 |
| propanoate fermentation to 2-methylbutanoate | 6 | 3 | 1 |
| Calvin-Benson-Bassham cycle | 13 | 10 | 2 |
| ethene biosynthesis III (microbes) | 7 | 6 | 1 |
| fatty acid β-oxidation I (generic) | 7 | 6 | 1 |
| fatty acid β-oxidation VI (mammalian peroxisome) | 7 | 5 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| benzoyl-CoA degradation I (aerobic) | 7 | 3 | 1 |
| L-valine degradation I | 8 | 6 | 1 |
| pyruvate fermentation to butanol I | 8 | 4 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 2 |
| oxygenic photosynthesis | 17 | 11 | 2 |
| sucrose biosynthesis I (from photosynthesis) | 9 | 7 | 1 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 3 |
| valproate β-oxidation | 9 | 6 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| chitin biosynthesis | 9 | 5 | 1 |
| phenylacetate degradation I (aerobic) | 9 | 4 | 1 |
| benzoate biosynthesis I (CoA-dependent, β-oxidative) | 9 | 3 | 1 |
| starch degradation II | 9 | 1 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 9 | 1 |
| peptidoglycan recycling II | 10 | 8 | 1 |
| L-glutamate degradation V (via hydroxyglutarate) | 10 | 6 | 1 |
| 3-phenylpropanoate degradation | 10 | 5 | 1 |
| starch biosynthesis | 10 | 5 | 1 |
| colanic acid building blocks biosynthesis | 11 | 11 | 1 |
| O-antigen building blocks biosynthesis (E. coli) | 11 | 10 | 1 |
| superpathway of phenylethylamine degradation | 11 | 6 | 1 |
| gallate degradation III (anaerobic) | 11 | 3 | 1 |
| Spodoptera littoralis pheromone biosynthesis | 22 | 4 | 2 |
| L-glutamate degradation VII (to butanoate) | 12 | 3 | 1 |
| ethene biosynthesis V (engineered) | 25 | 18 | 2 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 2 |
| superpathway of Clostridium acetobutylicum solventogenic fermentation | 13 | 5 | 1 |
| (4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) | 13 | 3 | 1 |
| superpathway of glyoxylate cycle and fatty acid degradation | 14 | 12 | 1 |
| peptidoglycan recycling I | 14 | 11 | 1 |
| docosahexaenoate biosynthesis III (6-desaturase, mammals) | 14 | 3 | 1 |
| Bifidobacterium shunt | 15 | 12 | 1 |
| L-tryptophan degradation III (eukaryotic) | 15 | 6 | 1 |
| glycerol degradation to butanol | 16 | 10 | 1 |
| crotonate fermentation (to acetate and cyclohexane carboxylate) | 16 | 4 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 7 | 1 |
| benzoate fermentation (to acetate and cyclohexane carboxylate) | 17 | 4 | 1 |
| 3-hydroxypropanoate/4-hydroxybutanate cycle | 18 | 8 | 1 |
| toluene degradation VI (anaerobic) | 18 | 4 | 1 |
| streptomycin biosynthesis | 18 | 2 | 1 |
| superpathway of anaerobic sucrose degradation | 19 | 14 | 1 |
| superpathway of dTDP-glucose-derived O-antigen building blocks biosynthesis | 19 | 5 | 1 |
| platensimycin biosynthesis | 26 | 6 | 1 |
| superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis | 33 | 12 | 1 |
| mycolate biosynthesis | 205 | 20 | 3 |
| superpathway of mycolate biosynthesis | 239 | 21 | 3 |