Experiment set11IT053 for Paraburkholderia graminis OAS925

Compare to:

histamine 2mM N source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + Histamine (2 mM)
Culturing: Burkholderia_OAS925_ML2, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Marta Torres on 26-Aug-21
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 5 genes in this experiment

For nitrogen source Histamine in Paraburkholderia graminis OAS925

For nitrogen source Histamine across organisms

SEED Subsystems

Subsystem #Specific
Lactate utilization 1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway 1
Lactose and Galactose Uptake and Utilization 1
N-linked Glycosylation in Bacteria 1
Rhamnose containing glycans 1
linker unit-arabinogalactan synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-N-acetyl-D-galactosamine biosynthesis I 1 1 1
UDP-α-D-galactose biosynthesis 1 1 1
UDP-α-D-galactofuranose biosynthesis 2 1 1
D-galactose detoxification 3 1 1
D-galactose degradation I (Leloir pathway) 5 3 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
stachyose degradation 7 2 1
UDP-sugars interconversion 9 3 1
teichuronic acid biosynthesis (B. subtilis 168) 9 2 1
colanic acid building blocks biosynthesis 11 11 1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 18 1 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 12 1