Experiment set11IT039 for Pseudomonas fluorescens FW300-N1B4

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nitrogen source Sodium nitrite 0.5mM

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + Sodium nitrite (0.5 mM), pH=7
Culturing: pseudo1_N1B4_ML1, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 11-Aug-20
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 3 genes in this experiment

For nitrogen source Sodium nitrite in Pseudomonas fluorescens FW300-N1B4

For nitrogen source Sodium nitrite across organisms

SEED Subsystems

Subsystem #Specific
Purine Utilization 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
pyruvate fermentation to (R)-acetoin II 2 1 1
pyruvate fermentation to (S)-acetoin 3 2 1
pyruvate fermentation to (R)-acetoin I 3 2 1
L-valine biosynthesis 4 4 1
pyruvate fermentation to isobutanol (engineered) 5 4 1
superpathway of (R,R)-butanediol biosynthesis 5 2 1
L-isoleucine biosynthesis IV 6 4 1
superpathway of 2,3-butanediol biosynthesis 6 2 1
L-isoleucine biosynthesis I (from threonine) 7 7 1
pyrroloquinoline quinone biosynthesis 7 4 1
L-isoleucine biosynthesis III 7 4 1
L-isoleucine biosynthesis II 8 5 1
superpathway of branched chain amino acid biosynthesis 17 17 2
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of L-threonine metabolism 18 12 1