Experiment set11IT022 for Burkholderia phytofirmans PsJN

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Sebacic acid carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Sebacic acid (10 mM)
Culturing: BFirm_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 3.5 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 4 genes in this experiment

For carbon source Sebacic acid in Burkholderia phytofirmans PsJN

For carbon source Sebacic acid across organisms

SEED Subsystems

Subsystem #Specific
Fatty Acid Biosynthesis FASII 1
Hydantoin metabolism 1
mycolic acid synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-idonate degradation 3 3 1
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) 12 12 3
gondoate biosynthesis (anaerobic) 4 4 1
palmitate biosynthesis III 29 28 7
tetradecanoate biosynthesis (mitochondria) 25 23 6
palmitate biosynthesis II (type II fatty acid synthase) 31 29 7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) 9 9 2
oleate biosynthesis IV (anaerobic) 14 13 3
superpathway of fatty acids biosynthesis (E. coli) 53 50 11
fatty acid elongation -- saturated 5 5 1
cis-vaccenate biosynthesis 5 5 1
superpathway of unsaturated fatty acids biosynthesis (E. coli) 20 19 4
8-amino-7-oxononanoate biosynthesis IV 5 4 1
superpathway of fatty acid biosynthesis II (plant) 43 38 8
8-amino-7-oxononanoate biosynthesis I 11 9 2
odd iso-branched-chain fatty acid biosynthesis 34 30 6
even iso-branched-chain fatty acid biosynthesis 34 30 6
anteiso-branched-chain fatty acid biosynthesis 34 30 6
(5Z)-dodecenoate biosynthesis I 6 5 1
stearate biosynthesis II (bacteria and plants) 6 5 1
(5Z)-dodecenoate biosynthesis II 6 4 1
stearate biosynthesis IV 6 4 1
petroselinate biosynthesis 6 2 1
streptorubin B biosynthesis 34 20 5
biotin biosynthesis I 15 13 2
superpathway of fatty acid biosynthesis I (E. coli) 16 14 2
ketogluconate metabolism 8 6 1
2-allylmalonyl-CoA biosynthesis 8 2 1
mycolate biosynthesis 205 25 5
superpathway of mycolate biosynthesis 239 26 5