Experiment set11IT019 for Pseudomonas fluorescens FW300-N2E2

Compare to:

RCH2 defined with Cobalt chloride hexahydrate 0.04 mM

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_3248 -6.1 -4.2 Probable transmembrane protein compare
Pf6N2E2_3060 -5.8 -3.2 Outer membrane protein H precursor compare
Pf6N2E2_4071 -5.7 -5.5 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_3782 -5.3 -8.9 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_5177 -4.9 -9.3 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_4187 -4.8 -12.5 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_71 -4.8 -3.3 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3610 -4.7 -3.2 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_64 -4.6 -2.8 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3383 -4.6 -7.0 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_77 -4.5 -3.1 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_2251 -4.5 -6.1 NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3) conserved
Pf6N2E2_3382 -4.5 -8.0 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_4072 -4.4 -7.4 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_5258 -4.4 -9.8 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_3783 -4.4 -5.0 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_3630 -4.3 -5.1 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3842 -4.3 -5.0 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_4597 -4.2 -5.7 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_5178 -4.2 -4.0 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_3384 -4.1 -5.6 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG compare
Pf6N2E2_2129 -4.1 -4.8 Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup compare
Pf6N2E2_2073 -4.1 -5.6 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3270 -4.0 -5.5 Hypothetical ATP-binding protein UPF0042, contains P-loop compare
Pf6N2E2_3752 -4.0 -19.0 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_3751 -4.0 -15.7 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_3381 -3.9 -8.6 Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE compare
Pf6N2E2_4564 -3.9 -10.8 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_4826 -3.8 -7.3 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3264 -3.8 -7.3 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_4596 -3.8 -7.6 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_6045 -3.7 -1.2 hypothetical protein compare
Pf6N2E2_3253 -3.7 -6.1 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_3932 -3.7 -5.7 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_5679 -3.7 -5.0 hypothetical protein compare
Pf6N2E2_4048 -3.7 -9.0 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_3841 -3.7 -3.6 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_66 -3.6 -9.8 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_5505 -3.6 -2.8 hypothetical protein compare
Pf6N2E2_4025 -3.5 -1.6 hypothetical protein compare
Pf6N2E2_2217 -3.4 -5.2 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_50 -3.4 -8.0 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_4279 -3.4 -13.4 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_4075 -3.3 -8.3 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_3261 -3.3 -10.3 hypothetical protein compare
Pf6N2E2_3661 -3.3 -3.9 hypothetical protein compare
Pf6N2E2_2186 -3.3 -6.4 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_2277 -3.3 -2.9 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_2013 -3.3 -1.0 hypothetical protein compare
Pf6N2E2_2121 -3.3 -2.1 hypothetical protein compare
Pf6N2E2_5704 -3.3 -4.4 Transcriptional regulatory protein PhoP compare
Pf6N2E2_3839 -3.2 -3.8 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3380 -3.2 -9.6 L-lactate permease compare
Pf6N2E2_5175 -3.2 -5.4 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_4083 -3.2 -3.7 hypothetical protein compare
Pf6N2E2_1673 -3.2 -2.4 Transcriptional regulator, TetR family compare
Pf6N2E2_4797 -3.2 -1.4 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_1083 -3.2 -1.7 hypothetical protein compare
Pf6N2E2_302 -3.1 -5.5 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3252 -3.1 -6.9 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_4825 -3.1 -7.9 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_5111 -3.1 -2.0 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_4277 -3.1 -12.3 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_4484 -3.1 -15.2 Ferric iron ABC transporter, permease protein conserved
Pf6N2E2_5316 -3.0 -2.1 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_2074 -3.0 -1.3 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_2258 -3.0 -9.1 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_4074 -3.0 -16.3 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_3820 -3.0 -1.9 hypothetical protein compare
Pf6N2E2_5578 -3.0 -7.7 Ferric iron ABC transporter, ATP-binding protein conserved
Pf6N2E2_3487 -3.0 -10.2 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_4614 -3.0 -3.5 twitching motility protein PilH compare
Pf6N2E2_3251 -3.0 -10.6 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_5257 -2.9 -8.6 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_4988 -2.9 -3.6 Antiholin-like protein LrgA compare
Pf6N2E2_3659 -2.9 -7.8 Protein fixF compare
Pf6N2E2_3940 -2.9 -9.5 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2605 -2.9 -16.3 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_5176 -2.8 -3.8 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3627 -2.8 -7.4 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1) compare
Pf6N2E2_6140 -2.8 -0.9 Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA compare
Pf6N2E2_4047 -2.8 -5.6 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_5520 -2.8 -8.0 Sensory box histidine kinase compare
Pf6N2E2_2218 -2.7 -4.1 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_4483 -2.7 -15.7 Ferric iron ABC transporter, iron-binding protein conserved
Pf6N2E2_3908 -2.7 -1.8 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2519 -2.7 -0.8 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_4453 -2.6 -13.3 Polyphosphate kinase (EC 2.7.4.1) compare
Pf6N2E2_5711 -2.6 -6.8 tRNA(Cytosine32)-2-thiocytidine synthetase compare
Pf6N2E2_4612 -2.6 -4.3 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_3666 -2.6 -2.5 InaA protein compare
Pf6N2E2_2286 -2.6 -2.1 FIG00953287: hypothetical protein compare
Pf6N2E2_3183 -2.6 -2.6 Believed to be involved in assembly of Fe-S clusters compare
Pf6N2E2_2465 -2.6 -4.6 Cys regulon transcriptional activator CysB compare
Pf6N2E2_4278 -2.6 -9.5 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_5666 -2.5 -9.0 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_3300 -2.5 -2.9 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_2261 -2.5 -4.1 VacJ-like lipoprotein precursor compare
Pf6N2E2_1676 -2.5 -2.6 conserved protein associated with acetyl-CoA C-acyltransferase compare
Pf6N2E2_2852 -2.5 -9.4 GGDEF domain/EAL domain protein compare
Pf6N2E2_1605 -2.5 -6.8 Carboxynorspermidine dehydrogenase, putative (EC 1.1.1.-) compare
Pf6N2E2_1081 -2.5 -1.5 probable membrane protein YPO3302 compare
Pf6N2E2_4974 -2.5 -5.8 DedA protein compare
Pf6N2E2_5723 -2.5 -7.2 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_5573 -2.5 -4.8 Glycerol-3-phosphate regulon repressor, DeoR family compare
Pf6N2E2_4092 -2.4 -4.3 FIG00954395: hypothetical protein compare
Pf6N2E2_2425 -2.4 -2.9 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3869 -2.4 -11.1 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_4989 -2.4 -4.7 LrgA-associated membrane protein LrgB compare
Pf6N2E2_5919 -2.4 -2.8 Flp pilus assembly protein TadD, contains TPR repeats compare
Pf6N2E2_4360 -2.4 -9.6 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_5545 -2.4 -3.5 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_2139 -2.3 -1.8 Paraquat-inducible protein A compare
Pf6N2E2_6064 -2.3 -6.0 2-methylaconitate isomerase compare
Pf6N2E2_1806 -2.3 -2.9 hypothetical protein compare
Pf6N2E2_4618 -2.3 -4.1 Chemotaxis signal transduction protein compare
Pf6N2E2_63 -2.3 -3.3 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_5618 -2.3 -9.5 Probable thiol oxidoreductase with 2 cytochrome c heme-binding sites conserved
Pf6N2E2_3170 -2.3 -6.4 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_5950 -2.2 -2.1 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_4476 -2.2 -4.3 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
Pf6N2E2_4517 -2.2 -4.6 Putrescine transport system permease protein PotI (TC 3.A.1.11.2) compare
Pf6N2E2_5728 -2.2 -3.6 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_2534 -2.2 -2.9 Cell division protein BolA compare
Pf6N2E2_638 -2.2 -2.1 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein compare
Pf6N2E2_5746 -2.2 -2.7 Cobalamin synthase compare
Pf6N2E2_760 -2.2 -10.4 Na+/H+ antiporter NhaB compare
Pf6N2E2_3079 -2.2 -7.4 Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA compare
Pf6N2E2_1606 -2.2 -4.8 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_5956 -2.1 -6.6 FIG004453: protein YceG like compare
Pf6N2E2_4889 -2.1 -6.2 Putative transport protein compare
Pf6N2E2_3656 -2.1 -14.7 Probable transcription regulator Mig-14 compare
Pf6N2E2_4120 -2.1 -11.8 Trk system potassium uptake protein TrkA compare
Pf6N2E2_4357 -2.1 -10.4 Nucleoside-diphosphate-sugar epimerases conserved
Pf6N2E2_2488 -2.1 -1.6 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_5006 -2.1 -4.0 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_2065 -2.1 -1.4 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_4615 -2.1 -1.6 type IV pili signal transduction protein PilI compare
Pf6N2E2_5188 -2.1 -2.0 Ribosomal-protein-S18p-alanine acetyltransferase (EC 2.3.1.-) compare
Pf6N2E2_4272 -2.1 -2.8 putative membrane protein compare
Pf6N2E2_4423 -2.1 -2.3 FIG00953342: hypothetical protein compare
Pf6N2E2_2606 -2.0 -8.4 hypothetical protein compare
Pf6N2E2_3226 -2.0 -3.3 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_3461 -2.0 -1.1 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_5756 -2.0 -3.5 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_5616 -2.0 -5.5 outer membrane component of uptake system, probably for ferrous iron (from data) conserved
Pf6N2E2_2904 -2.0 -2.1 Hypothetical protein DUF454 compare
Pf6N2E2_3088 -2.0 -1.5 hypothetical protein compare
Pf6N2E2_4413 -2.0 -8.3 FIG004064: hypothetical protein compare
Pf6N2E2_2418 -2.0 -2.4 DNA-binding response regulator, LuxR family compare
Pf6N2E2_2375 -2.0 -2.1 Response regulator NasT compare
Pf6N2E2_5014 -2.0 -0.6 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_4987 -2.0 -0.6 MaoC-like domain protein compare
Pf6N2E2_3763 -2.0 -1.1 hypothetical protein compare
Pf6N2E2_3132 -2.0 -3.4 FIG00954548: hypothetical protein compare
Pf6N2E2_3534 -1.9 -2.8 Cobalamin biosynthesis protein CobG compare
Pf6N2E2_3612 -1.9 -4.1 HflK protein compare
Pf6N2E2_3761 -1.9 -13.0 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_3477 -1.9 -0.9 2-Oxobutyrate oxidase, putative compare
Pf6N2E2_4416 -1.9 -1.8 Tyrosine recombinase XerC compare
Pf6N2E2_895 -1.9 -4.7 Transcriptional regulator, HxlR family compare
Pf6N2E2_4067 -1.9 -8.0 Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3) compare
Pf6N2E2_4817 -1.9 -12.3 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_5780 -1.9 -6.5 Periplasmic protease compare
Pf6N2E2_1477 -1.9 -4.0 hypothetical protein compare
Pf6N2E2_350 -1.9 -1.1 Gfa-like protein compare
Pf6N2E2_1034 -1.9 -1.6 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_2045 -1.9 -3.7 Peptidyl-prolyl cis-trans isomerase PpiC (EC 5.2.1.8) compare
Pf6N2E2_51 -1.9 -5.2 hypothetical protein compare
Pf6N2E2_2675 -1.9 -1.0 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_3062 -1.8 -3.2 Membrane-associated zinc metalloprotease compare
Pf6N2E2_5659 -1.8 -13.5 Acetyl-coenzyme A synthetase (EC 6.2.1.1) compare
Pf6N2E2_1031 -1.8 -3.0 Alkanesulfonates transport system permease protein compare
Pf6N2E2_3323 -1.8 -2.1 hypothetical protein compare
Pf6N2E2_3933 -1.8 -5.3 D-2-hydroxyglutarate dehydrogenase compare
Pf6N2E2_3868 -1.8 -4.2 Transcriptional regulator, LysR family compare
Pf6N2E2_3658 -1.8 -6.5 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_2061 -1.8 -4.4 CobW GTPase involved in cobalt insertion for B12 biosynthesis compare
Pf6N2E2_2510 -1.8 -1.2 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_3833 -1.8 -3.3 hypothetical protein compare
Pf6N2E2_1239 -1.7 -5.6 Putative TEGT family carrier/transport protein compare
Pf6N2E2_1381 -1.7 -5.5 Aldehyde dehydrogenase (EC 1.2.1.3) compare
Pf6N2E2_3351 -1.7 -2.0 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_42 -1.7 -3.7 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_3040 -1.7 -5.9 5-nucleotidase SurE (EC 3.1.3.5) compare
Pf6N2E2_3611 -1.7 -2.0 HflC protein compare
Pf6N2E2_4422 -1.7 -1.9 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_2646 -1.7 -0.9 FIG00953416: hypothetical protein compare
Pf6N2E2_4049 -1.7 -7.5 Glycine cleavage system transcriptional activator compare
Pf6N2E2_4516 -1.7 -7.4 Putrescine transport system permease protein PotH (TC 3.A.1.11.2) compare
Pf6N2E2_4153 -1.7 -4.4 Sensory box histidine kinase compare
Pf6N2E2_2588 -1.7 -2.5 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_2885 -1.7 -4.9 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_2574 -1.7 -1.8 Flagellar synthesis regulator FleN compare
Pf6N2E2_2681 -1.7 -2.0 3-phosphoglycerate kinase compare
Pf6N2E2_2391 -1.7 -6.6 FIG137360: hypothetical protein compare
Pf6N2E2_4160 -1.7 -1.3 Cytochrome c5 compare
Pf6N2E2_620 -1.7 -6.3 BatD compare
Pf6N2E2_3826 -1.7 -4.4 Nitrogen regulation protein NR(I) compare
Pf6N2E2_3373 -1.7 -4.4 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare


Specific Phenotypes

For 41 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms