Experiment set11IT018 for Burkholderia phytofirmans PsJN

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Sodium succinate dibasic hexahydrate carbon source

Group: carbon source
Media: MOPS minimal media_noCarbon + Sodium succinate dibasic hexahydrate (10 mM)
Culturing: BFirm_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 1.7 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate

Specific Phenotypes

For 12 genes in this experiment

For carbon source Sodium succinate dibasic hexahydrate in Burkholderia phytofirmans PsJN

For carbon source Sodium succinate dibasic hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Biotin biosynthesis 1
Ribosome activity modulation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 1
biotin biosynthesis I 15 13 1