Experiment set11IT016 for Pseudomonas fluorescens FW300-N2E2

Compare to:

RCH2 defined with Cobalt chloride hexahydrate 0.02 mM

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_3783 -6.1 -4.2 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_3782 -6.1 -9.3 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3842 -5.9 -4.1 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_4048 -5.7 -5.6 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_2129 -5.7 -3.9 Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup compare
Pf6N2E2_5177 -5.5 -9.8 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3381 -5.5 -7.5 Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE compare
Pf6N2E2_3248 -5.2 -5.5 Probable transmembrane protein compare
Pf6N2E2_3630 -5.2 -5.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3382 -5.1 -8.6 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_4826 -4.9 -7.4 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3253 -4.8 -5.7 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4279 -4.8 -17.0 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_4564 -4.8 -11.2 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_4597 -4.8 -6.6 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_4825 -4.8 -5.7 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3751 -4.7 -15.3 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4047 -4.7 -3.2 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_3610 -4.6 -4.5 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_71 -4.5 -6.1 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_2073 -4.4 -7.9 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_77 -4.4 -4.3 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4596 -4.4 -8.4 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3752 -4.4 -21.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_3384 -4.2 -6.3 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG compare
Pf6N2E2_3841 -4.1 -4.9 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3839 -4.0 -3.9 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3380 -3.9 -13.1 L-lactate permease compare
Pf6N2E2_237 -3.9 -2.6 hypothetical protein compare
Pf6N2E2_5175 -3.8 -6.8 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_1083 -3.7 -2.3 hypothetical protein compare
Pf6N2E2_3383 -3.7 -7.0 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_4277 -3.7 -12.7 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_302 -3.7 -6.8 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3940 -3.7 -10.4 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4278 -3.6 -10.3 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_5176 -3.5 -4.2 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_66 -3.5 -8.9 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_2899 -3.4 -6.1 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_64 -3.4 -2.7 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3932 -3.4 -3.7 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_4072 -3.4 -10.8 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_50 -3.4 -8.4 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_63 -3.3 -4.6 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_2074 -3.3 -1.7 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_5728 -3.3 -7.4 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_3627 -3.3 -8.7 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1) compare
Pf6N2E2_5723 -3.1 -8.5 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_2440 -3.1 -1.9 Free methionine-(R)-sulfoxide reductase, contains GAF domain compare
Pf6N2E2_5150 -3.0 -6.9 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_4416 -3.0 -2.1 Tyrosine recombinase XerC compare
Pf6N2E2_3826 -3.0 -5.5 Nitrogen regulation protein NR(I) compare
Pf6N2E2_2984 -3.0 -2.8 LysR family transcriptional regulator PA3398 compare
Pf6N2E2_4797 -2.9 -2.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_3251 -2.9 -11.9 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_2465 -2.9 -5.0 Cys regulon transcriptional activator CysB compare
Pf6N2E2_309 -2.9 -1.4 FIG00957702: hypothetical protein compare
Pf6N2E2_3908 -2.8 -4.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_5679 -2.8 -5.0 hypothetical protein compare
Pf6N2E2_3252 -2.8 -7.2 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_4272 -2.7 -3.7 putative membrane protein compare
Pf6N2E2_5666 -2.7 -11.2 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_2681 -2.7 -3.1 3-phosphoglycerate kinase compare
Pf6N2E2_5704 -2.7 -4.2 Transcriptional regulatory protein PhoP compare
Pf6N2E2_4601 -2.6 -2.2 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_3461 -2.6 -1.6 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_3763 -2.6 -1.6 hypothetical protein compare
Pf6N2E2_4394 -2.6 -5.7 Putrescine utilization regulator compare
Pf6N2E2_2277 -2.6 -3.2 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_338 -2.5 -1.8 Transcriptional regulator, AraC family compare
Pf6N2E2_5841 -2.5 -0.9 GlcG protein compare
Pf6N2E2_5160 -2.4 -1.2 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_5794 -2.4 -1.4 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_5014 -2.3 -0.8 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_760 -2.3 -12.6 Na+/H+ antiporter NhaB compare
Pf6N2E2_3612 -2.3 -4.3 HflK protein compare
Pf6N2E2_1673 -2.3 -1.8 Transcriptional regulator, TetR family compare
Pf6N2E2_5258 -2.3 -8.8 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_3170 -2.3 -6.4 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_3477 -2.2 -1.1 2-Oxobutyrate oxidase, putative compare
Pf6N2E2_2518 -2.2 -5.0 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_5574 -2.2 -8.0 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_2640 -2.1 -1.6 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_3261 -2.1 -8.2 hypothetical protein compare
Pf6N2E2_1391 -2.0 -3.0 DNA-binding response regulator, LuxR family compare
Pf6N2E2_4414 -2.0 -1.4 FIG00953808: hypothetical protein compare
Pf6N2E2_5153 -2.0 -5.9 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_4083 -1.9 -2.9 hypothetical protein compare
Pf6N2E2_2022 -1.9 -2.2 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_4599 -1.9 -3.1 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_4579 -1.9 -1.3 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_4762 -1.9 -2.2 Biotin synthesis protein BioH compare
Pf6N2E2_2261 -1.9 -5.7 VacJ-like lipoprotein precursor compare
Pf6N2E2_4372 -1.9 -1.3 FIG00460773: hypothetical protein compare
Pf6N2E2_5536 -1.9 -1.2 Holliday junction DNA helicase RuvA compare
Pf6N2E2_4364 -1.9 -3.0 Protein YicC compare
Pf6N2E2_4476 -1.8 -3.7 5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) compare
Pf6N2E2_3899 -1.8 -8.1 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_2418 -1.8 -2.7 DNA-binding response regulator, LuxR family compare
Pf6N2E2_3938 -1.8 -12.1 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_4206 -1.8 -1.7 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_1705 -1.8 -2.5 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_2274 -1.8 -4.1 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_3611 -1.8 -1.7 HflC protein compare
Pf6N2E2_3568 -1.8 -1.7 Unsaturated fatty acid biosythesis repressor FabR, TetR family compare
Pf6N2E2_4074 -1.8 -14.5 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_1932 -1.8 -4.0 Transcriptional regulator, IclR family compare
Pf6N2E2_2588 -1.8 -3.1 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_70 -1.8 -4.3 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_1095 -1.7 -2.2 Opine oxidase subunit C compare
Pf6N2E2_4162 -1.7 -4.6 DNA polymerase I (EC 2.7.7.7) compare
Pf6N2E2_2746 -1.7 -4.9 Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor compare
Pf6N2E2_2513 -1.7 -2.0 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5575 -1.7 -2.3 Glycerol uptake facilitator protein compare
Pf6N2E2_4077 -1.7 -2.1 cytosolic long-chain acyl-CoA thioester hydrolase family protein compare
Pf6N2E2_5560 -1.7 -3.0 Sigma-54 dependent transcriptional regulator/sensory box protein compare
Pf6N2E2_5257 -1.7 -9.0 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_601 -1.7 -3.6 GlpG protein (membrane protein of glp regulon) compare
Pf6N2E2_5667 -1.7 -8.2 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_6093 -1.7 -2.7 hypothetical protein compare
Pf6N2E2_5178 -1.7 -3.3 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_3258 -1.7 -3.1 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_2139 -1.7 -2.3 Paraquat-inducible protein A compare
Pf6N2E2_1239 -1.7 -6.6 Putative TEGT family carrier/transport protein compare
Pf6N2E2_3055 -1.6 -2.4 Ribonuclease HII (EC 3.1.26.4) compare
Pf6N2E2_2121 -1.6 -1.6 hypothetical protein compare
Pf6N2E2_2885 -1.6 -5.7 Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49) compare
Pf6N2E2_5950 -1.6 -2.7 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_2986 -1.6 -2.3 Transcriptional regulator, LuxR family compare
Pf6N2E2_5156 -1.6 -1.8 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2133 -1.6 -5.0 Putative sulfate permease compare
Pf6N2E2_814 -1.6 -3.5 hypothetical protein compare
Pf6N2E2_3825 -1.6 -3.8 Nitrogen regulation protein NtrB (EC 2.7.13.3) compare
Pf6N2E2_4049 -1.6 -7.7 Glycine cleavage system transcriptional activator compare
Pf6N2E2_4071 -1.6 -4.0 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_5520 -1.6 -7.3 Sensory box histidine kinase compare
Pf6N2E2_3933 -1.6 -7.2 D-2-hydroxyglutarate dehydrogenase compare
Pf6N2E2_3027 -1.5 -0.7 Baseplate assembly protein V compare
Pf6N2E2_1179 -1.5 -3.7 hypothetical protein compare
Pf6N2E2_2510 -1.5 -2.6 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_1056 -1.5 -0.7 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_649 -1.5 -2.8 General secretion pathway protein L compare
Pf6N2E2_3351 -1.5 -3.1 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_5676 -1.5 -3.9 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_3537 -1.5 -3.0 Cobalt-precorrin-6x reductase (EC 1.3.1.54) compare
Pf6N2E2_5571 -1.5 -1.5 hypothetical protein compare
Pf6N2E2_4187 -1.5 -6.9 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_2823 -1.4 -4.9 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_3072 -1.4 -8.6 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_5103 -1.4 -1.4 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_2752 -1.4 -5.1 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_51 -1.4 -5.9 hypothetical protein compare
Pf6N2E2_1968 -1.4 -2.2 Cobalt-zinc-cadmium resistance protein compare
Pf6N2E2_3504 -1.4 -6.9 Biosynthetic arginine decarboxylase (EC 4.1.1.19) compare
Pf6N2E2_3264 -1.4 -6.4 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_1884 -1.4 -4.0 oxidoreductase, short-chain dehydrogenase/reductase family compare
Pf6N2E2_384 -1.4 -3.0 Glycine cleavage system transcriptional activator compare
Pf6N2E2_5780 -1.4 -6.3 Periplasmic protease compare
Pf6N2E2_3505 -1.4 -0.7 Translation initiation factor SUI1-related protein compare
Pf6N2E2_987 -1.3 -1.2 hypothetical protein compare
Pf6N2E2_5452 -1.3 -1.8 hypothetical protein compare
Pf6N2E2_3941 -1.3 -1.9 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_3095 -1.3 -0.4 hypothetical protein compare
Pf6N2E2_3346 -1.3 -5.1 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_2088 -1.3 -1.7 SOS-response repressor and protease LexA (EC 3.4.21.88) compare
Pf6N2E2_5428 -1.3 -5.4 DNA recombination and repair protein RecO compare
Pf6N2E2_5001 -1.3 -1.4 D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) compare
Pf6N2E2_241 -1.3 -1.2 hypothetical protein compare
Pf6N2E2_1000 -1.3 -4.5 hypothetical protein compare
Pf6N2E2_3770 -1.3 -1.6 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_55 -1.3 -1.7 Probable hydrolase compare
Pf6N2E2_1987 -1.3 -1.4 hypothetical protein compare
Pf6N2E2_2825 -1.2 -4.3 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_1065 -1.2 -2.9 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_1935 -1.2 -3.3 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_478 -1.2 -2.5 Dihydrolipoyl dehydrogenase (EC 1.8.1.4) (from data) compare
Pf6N2E2_4517 -1.2 -4.0 Putrescine transport system permease protein PotI (TC 3.A.1.11.2) compare
Pf6N2E2_2675 -1.2 -1.0 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_5742 -1.2 -4.8 Cobyric acid synthase (EC 6.3.5.10) compare
Pf6N2E2_74 -1.2 -2.3 DedD protein compare
Pf6N2E2_16 -1.2 -1.5 hypothetical protein compare
Pf6N2E2_4342 -1.2 -3.1 Protein phosphatase ImpM compare
Pf6N2E2_2152 -1.2 -0.8 hypothetical protein compare
Pf6N2E2_3300 -1.2 -2.3 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_2258 -1.2 -6.7 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_423 -1.2 -1.0 Putative phosphatase YfbT compare
Pf6N2E2_2200 -1.2 -0.5 RNA polymerase sigma-54 factor RpoN compare
Pf6N2E2_4352 -1.2 -0.8 ATP-dependent DNA helicase RecG (EC 3.6.1.-) compare
Pf6N2E2_1606 -1.2 -4.2 Carboxynorspermidine decarboxylase, putative (EC 4.1.1.-) compare
Pf6N2E2_5579 -1.2 -4.3 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_3460 -1.2 -0.7 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_2480 -1.2 -0.8 FIG00957636: hypothetical protein compare
Pf6N2E2_4988 -1.2 -2.8 Antiholin-like protein LrgA compare
Pf6N2E2_2519 -1.2 -0.6 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_1797 -1.1 -1.4 Transcriptional regulator compare
Pf6N2E2_3171 -1.1 -1.0 hypothetical protein compare
Pf6N2E2_1148 -1.1 -3.1 Butyryl-CoA dehydrogenase (EC 1.3.99.2) compare
Pf6N2E2_3901 -1.1 -1.5 Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit (S4 paralog) compare
Pf6N2E2_5342 -1.1 -1.7 21 kDa hemolysin precursor compare
Pf6N2E2_5190 -1.1 -5.5 Carbonic anhydrase (EC 4.2.1.1) compare


Specific Phenotypes

For 3 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms