Experiment set11IT015 for Pseudomonas fluorescens FW300-N2E2

Compare to:

RCH2 defined with Nickel (II) chloride hexahydrate 0.04 mM

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_3248 -5.6 -5.5 Probable transmembrane protein compare
Pf6N2E2_4826 -5.6 -5.5 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3382 -5.5 -6.5 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_2129 -5.4 -3.7 Predicted dye-decolorizing peroxidase (DyP), YfeX-like subgroup compare
Pf6N2E2_3782 -5.4 -9.5 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3383 -5.3 -6.3 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_4048 -5.0 -8.3 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_3630 -4.9 -4.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5177 -4.8 -11.8 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_4825 -4.6 -5.5 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3751 -4.6 -14.6 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4279 -4.6 -16.4 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_3381 -4.6 -9.2 Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE compare
Pf6N2E2_4278 -4.6 -13.4 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_4564 -4.5 -10.9 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_3752 -4.5 -20.8 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_4597 -4.5 -6.8 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3842 -4.4 -5.8 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_2281 -4.3 -3.5 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_3783 -4.3 -7.0 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_3610 -4.1 -4.9 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_3380 -4.0 -11.8 L-lactate permease compare
Pf6N2E2_4601 -3.9 -2.3 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_77 -3.8 -5.0 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3839 -3.8 -3.7 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_3253 -3.8 -6.3 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_71 -3.8 -5.7 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3612 -3.8 -3.7 HflK protein compare
Pf6N2E2_4047 -3.8 -5.8 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_64 -3.8 -2.9 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_4596 -3.7 -9.3 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_50 -3.6 -7.0 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_4072 -3.6 -9.4 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_4071 -3.6 -5.8 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_3060 -3.6 -2.1 Outer membrane protein H precursor compare
Pf6N2E2_4277 -3.6 -11.4 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_2465 -3.6 -5.5 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2073 -3.4 -8.7 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_4797 -3.4 -1.6 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_3841 -3.4 -5.6 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3932 -3.4 -6.5 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_5175 -3.4 -7.5 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3696 -3.3 -1.2 hypothetical protein compare
Pf6N2E2_3627 -3.3 -9.5 Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1) compare
Pf6N2E2_3384 -3.3 -4.5 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG compare
Pf6N2E2_3252 -3.3 -7.3 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_302 -3.3 -6.2 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3264 -3.3 -7.2 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_5176 -3.2 -4.4 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_66 -3.2 -8.0 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_2074 -3.1 -1.5 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_3940 -3.1 -9.8 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3096 -3.1 -1.1 Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_5640 -2.9 -2.1 FIG00955597: hypothetical protein compare
Pf6N2E2_1673 -2.9 -2.9 Transcriptional regulator, TetR family compare
Pf6N2E2_5723 -2.9 -7.5 GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2) compare
Pf6N2E2_3074 -2.9 -1.2 FIG00960973: hypothetical protein compare
Pf6N2E2_3908 -2.8 -3.3 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_5728 -2.8 -5.2 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_5704 -2.8 -4.7 Transcriptional regulatory protein PhoP compare
Pf6N2E2_2261 -2.8 -4.5 VacJ-like lipoprotein precursor compare
Pf6N2E2_3251 -2.7 -10.2 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_2286 -2.6 -2.4 FIG00953287: hypothetical protein compare
Pf6N2E2_5666 -2.6 -11.1 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_2588 -2.6 -3.8 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_63 -2.5 -4.7 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_1239 -2.5 -7.5 Putative TEGT family carrier/transport protein compare
Pf6N2E2_5679 -2.5 -5.2 hypothetical protein compare
Pf6N2E2_5153 -2.5 -5.6 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_3461 -2.5 -1.5 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_4074 -2.5 -17.3 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_3763 -2.5 -1.4 hypothetical protein compare
Pf6N2E2_2899 -2.4 -4.5 NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) compare
Pf6N2E2_5841 -2.4 -0.8 GlcG protein compare
Pf6N2E2_4187 -2.4 -12.1 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_4416 -2.3 -2.2 Tyrosine recombinase XerC compare
Pf6N2E2_3261 -2.3 -9.8 hypothetical protein compare
Pf6N2E2_3826 -2.3 -5.5 Nitrogen regulation protein NR(I) compare
Pf6N2E2_3941 -2.3 -1.5 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_5919 -2.3 -2.9 Flp pilus assembly protein TadD, contains TPR repeats compare
Pf6N2E2_2681 -2.3 -3.1 3-phosphoglycerate kinase compare
Pf6N2E2_5014 -2.2 -0.8 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_760 -2.2 -10.7 Na+/H+ antiporter NhaB compare
Pf6N2E2_2496 -2.2 -1.8 FIG00956575: hypothetical protein compare
Pf6N2E2_906 -2.2 -2.3 ISPpu14, transposase Orf2 compare
Pf6N2E2_5667 -2.1 -9.3 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_3170 -2.1 -5.5 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_1797 -2.1 -1.9 Transcriptional regulator compare
Pf6N2E2_5574 -2.1 -6.7 Glycerol kinase (EC 2.7.1.30) compare
Pf6N2E2_5178 -2.1 -2.8 CDP-diacylglycerol--serine O-phosphatidyltransferase (EC 2.7.8.8) compare
Pf6N2E2_4394 -2.1 -4.8 Putrescine utilization regulator compare
Pf6N2E2_5505 -2.1 -2.5 hypothetical protein compare
Pf6N2E2_2605 -2.0 -15.3 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_3326 -2.0 -1.2 hypothetical protein compare
Pf6N2E2_5103 -2.0 -1.4 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_309 -2.0 -1.3 FIG00957702: hypothetical protein compare
Pf6N2E2_2823 -2.0 -5.6 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_1776 -2.0 -2.6 Chemotaxis protein CheD compare
Pf6N2E2_4988 -2.0 -4.1 Antiholin-like protein LrgA compare
Pf6N2E2_4057 -2.0 -0.7 lipoprotein, putative compare
Pf6N2E2_2251 -1.9 -4.9 NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3) conserved
Pf6N2E2_2274 -1.9 -3.7 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_3351 -1.9 -3.1 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_5257 -1.9 -9.9 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_5961 -1.9 -8.5 Transcriptional regulator, TetR family compare
Pf6N2E2_5342 -1.9 -1.3 21 kDa hemolysin precursor compare
Pf6N2E2_4414 -1.9 -1.3 FIG00953808: hypothetical protein compare
Pf6N2E2_1179 -1.9 -3.3 hypothetical protein compare
Pf6N2E2_2065 -1.8 -2.0 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_4599 -1.8 -2.7 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_4579 -1.8 -1.2 Ribosomal RNA small subunit methyltransferase D (EC 2.1.1.-) compare
Pf6N2E2_2133 -1.8 -5.7 Putative sulfate permease compare
Pf6N2E2_3938 -1.8 -10.3 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_5258 -1.8 -7.2 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_3300 -1.8 -3.2 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_3876 -1.8 -3.1 Agmatine deiminase (EC 3.5.3.12) compare
Pf6N2E2_1932 -1.8 -3.9 Transcriptional regulator, IclR family compare
Pf6N2E2_4075 -1.7 -6.1 Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1) compare
Pf6N2E2_3499 -1.7 -2.2 Permeases of the major facilitator superfamily compare
Pf6N2E2_5156 -1.7 -1.2 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_2824 -1.7 -7.1 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_5160 -1.7 -1.2 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_5536 -1.7 -1.1 Holliday junction DNA helicase RuvA compare
Pf6N2E2_2606 -1.7 -6.6 hypothetical protein compare
Pf6N2E2_3770 -1.7 -1.2 ATP-dependent hsl protease ATP-binding subunit HslU compare
Pf6N2E2_4049 -1.7 -7.3 Glycine cleavage system transcriptional activator compare
Pf6N2E2_5575 -1.7 -1.2 Glycerol uptake facilitator protein compare
Pf6N2E2_3933 -1.7 -5.6 D-2-hydroxyglutarate dehydrogenase compare
Pf6N2E2_2852 -1.7 -7.5 GGDEF domain/EAL domain protein compare
Pf6N2E2_3786 -1.7 -7.1 Twin-arginine translocation protein TatC compare
Pf6N2E2_288 -1.7 -1.1 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_5669 -1.7 -5.8 Succinylarginine dihydrolase (EC 3.5.3.23) compare
Pf6N2E2_2152 -1.6 -0.7 hypothetical protein compare
Pf6N2E2_4762 -1.6 -2.1 Biotin synthesis protein BioH compare
Pf6N2E2_344 -1.6 -2.5 hypothetical protein compare
Pf6N2E2_5024 -1.6 -2.3 Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7) compare
Pf6N2E2_895 -1.6 -4.3 Transcriptional regulator, HxlR family compare
Pf6N2E2_4272 -1.6 -2.8 putative membrane protein compare
Pf6N2E2_1248 -1.6 -1.3 MutT/nudix family protein compare
Pf6N2E2_2022 -1.6 -2.3 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_4763 -1.6 -5.3 Biotin synthesis protein BioC compare
Pf6N2E2_4013 -1.6 -7.1 5-aminovalerate transaminase (EC 2.6.1.48) (from data) compare
Pf6N2E2_888 -1.6 -2.2 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_3634 -1.6 -1.9 Transporter compare
Pf6N2E2_3072 -1.6 -7.3 [Protein-PII] uridylyltransferase (EC 2.7.7.59) compare
Pf6N2E2_5780 -1.6 -6.4 Periplasmic protease compare
Pf6N2E2_1352 -1.6 -2.1 Cation transport ATPase compare
Pf6N2E2_3003 -1.6 -2.0 Bacteriophage protein GP46 compare
Pf6N2E2_2586 -1.5 -2.1 Hpt domain protein compare
Pf6N2E2_3260 -1.5 -3.2 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_3885 -1.5 -1.0 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_3820 -1.5 -2.3 hypothetical protein compare
Pf6N2E2_2825 -1.5 -4.3 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_2707 -1.5 -2.8 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_3480 -1.5 -0.7 Transcriptional regulator, ArsR family compare
Pf6N2E2_1499 -1.5 -3.1 ABC transporter amino acid-binding protein compare
Pf6N2E2_3017 -1.5 -3.5 hypothetical protein compare
Pf6N2E2_5950 -1.5 -2.7 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_3258 -1.5 -2.9 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_3088 -1.5 -1.3 hypothetical protein compare
Pf6N2E2_5328 -1.5 -3.3 FIG00958649: hypothetical protein compare
Pf6N2E2_3349 -1.5 -3.4 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_2810 -1.5 -3.0 Cardiolipin synthetase (EC 2.7.8.-) compare
Pf6N2E2_2938 -1.4 -2.3 Outer membrane lipoprotein compare
Pf6N2E2_2526 -1.4 -4.7 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_2277 -1.4 -2.1 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_4298 -1.4 -1.8 Uncharacterized protein ImpB compare
Pf6N2E2_5733 -1.4 -3.2 NLP/P60 family protein compare
Pf6N2E2_5676 -1.4 -3.5 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_5748 -1.4 -1.6 Transcriptional activator protein LuxR compare
Pf6N2E2_58 -1.4 -2.0 tRNA-dihydrouridine synthase C (EC 1.-.-.-) compare
Pf6N2E2_3257 -1.4 -0.9 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_1749 -1.4 -2.1 Cyn operon transcriptional activator compare
Pf6N2E2_5794 -1.4 -1.3 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_70 -1.4 -3.8 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_1040 -1.4 -1.7 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_4391 -1.4 -4.7 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf6N2E2_2072 -1.4 -3.4 hypothetical protein compare
Pf6N2E2_2791 -1.4 -1.5 FIG00954883: hypothetical protein compare
Pf6N2E2_4764 -1.3 -3.6 Dethiobiotin synthetase (EC 6.3.3.3) compare
Pf6N2E2_2510 -1.3 -2.4 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_248 -1.3 -4.6 hypothetical protein compare
Pf6N2E2_170 -1.3 -1.4 lipoprotein, putative compare
Pf6N2E2_659 -1.3 -2.2 Transcriptional regulatory protein ompR compare
Pf6N2E2_5455 -1.3 -2.8 hypothetical protein compare
Pf6N2E2_3589 -1.3 -1.5 Lysine decarboxylase family compare
Pf6N2E2_2253 -1.3 -2.1 hypothetical protein compare
Pf6N2E2_1656 -1.3 -1.1 hypothetical protein compare
Pf6N2E2_1545 -1.3 -2.5 N-carbamoylputrescine amidase (EC 3.5.1.53) compare
Pf6N2E2_1099 -1.3 -2.1 Hypothetical protein GlcG in glycolate utilization operon compare
Pf6N2E2_3840 -1.3 -2.2 FIG00956267: hypothetical protein compare
Pf6N2E2_2574 -1.3 -1.4 Flagellar synthesis regulator FleN compare
Pf6N2E2_4364 -1.3 -2.1 Protein YicC compare
Pf6N2E2_2068 -1.3 -4.2 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) compare
Pf6N2E2_1717 -1.3 -5.4 Anthranilate synthase, aminase component (EC 4.1.3.27) compare
Pf6N2E2_1044 -1.3 -3.2 hypothetical protein compare
Pf6N2E2_1312 -1.3 -2.3 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS) compare
Pf6N2E2_2675 -1.3 -0.9 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_5150 -1.3 -3.2 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_2827 -1.3 -1.7 hypothetical protein compare


Specific Phenotypes

For 12 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Nickel (II) chloride hexahydrate across organisms