Experiment set11IT008 for Pseudomonas fluorescens FW300-N2E2

Compare to:

RCH2 defined with Zinc sulfate heptahydrate 0.032 mM

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_64 -4.9 -5.2 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_4596 -4.9 -9.9 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_2574 -4.7 -3.2 Flagellar synthesis regulator FleN compare
Pf6N2E2_4187 -4.7 -11.4 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_3751 -4.5 -15.8 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_4826 -4.4 -9.4 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3948 -4.3 -11.8 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_4279 -4.3 -14.9 Pyruvate carboxyl transferase subunit B (EC 6.4.1.1) compare
Pf6N2E2_4597 -4.3 -10.6 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_2074 -4.2 -2.8 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_3752 -4.2 -23.1 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_4048 -4.1 -9.6 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_5720 -4.1 -13.5 BarA sensory histidine kinase (= VarS = GacS) compare
Pf6N2E2_2217 -4.1 -5.5 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_71 -4.0 -7.3 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3248 -4.0 -6.1 Probable transmembrane protein compare
Pf6N2E2_5006 -4.0 -6.6 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_5176 -4.0 -8.1 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3842 -4.0 -5.7 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_3253 -4.0 -8.4 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_2022 -4.0 -3.9 BarA-associated response regulator UvrY (= GacA = SirA) compare
Pf6N2E2_3783 -3.9 -7.5 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4825 -3.9 -10.4 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_5679 -3.9 -5.3 hypothetical protein compare
Pf6N2E2_3630 -3.9 -7.2 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_4564 -3.8 -11.5 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_5177 -3.8 -11.8 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3383 -3.7 -7.1 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_2822 -3.7 -8.4 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_3461 -3.7 -2.5 Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) compare
Pf6N2E2_5014 -3.7 -1.7 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_2073 -3.7 -9.7 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3382 -3.6 -8.0 Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF compare
Pf6N2E2_4473 -3.6 -11.9 HlyD family secretion protein compare
Pf6N2E2_4472 -3.6 -17.1 ABC-type multidrug transport system, permease component compare
Pf6N2E2_2752 -3.6 -5.4 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_5175 -3.5 -10.9 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_3839 -3.5 -5.3 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_2513 -3.5 -5.7 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_4071 -3.4 -5.3 Phosphate transport system regulatory protein PhoU compare
Pf6N2E2_4047 -3.4 -6.8 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_3645 -3.4 -10.5 Type I secretion outer membrane protein, TolC precursor compare
Pf6N2E2_66 -3.4 -10.4 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_2323 -3.3 -3.2 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_3381 -3.3 -8.0 Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE compare
Pf6N2E2_3661 -3.3 -9.8 hypothetical protein compare
Pf6N2E2_3932 -3.3 -5.4 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_77 -3.2 -6.1 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_3252 -3.1 -7.8 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_3264 -3.1 -4.5 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3037 -3.0 -13.7 RNA polymerase sigma factor RpoS compare
Pf6N2E2_3782 -3.0 -6.1 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_2277 -3.0 -3.5 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_2510 -3.0 -6.8 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_4278 -2.9 -12.2 Pyruvate carboxyl transferase subunit A (EC 6.4.1.1) compare
Pf6N2E2_3380 -2.8 -7.7 L-lactate permease compare
Pf6N2E2_3610 -2.8 -4.9 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_3841 -2.7 -4.8 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_3351 -2.7 -5.4 tRNA pseudouridine synthase B (EC 4.2.1.70) compare
Pf6N2E2_2218 -2.7 -5.0 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_4471 -2.7 -9.4 ABC-type multidrug transport system, permease component compare
Pf6N2E2_3940 -2.7 -10.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3908 -2.6 -4.2 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_3876 -2.5 -3.8 Agmatine deiminase (EC 3.5.3.12) compare
Pf6N2E2_63 -2.5 -4.0 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_3384 -2.5 -6.0 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG compare
Pf6N2E2_4797 -2.5 -2.0 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_2261 -2.5 -5.2 VacJ-like lipoprotein precursor compare
Pf6N2E2_5242 -2.5 -1.7 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_4277 -2.4 -10.4 LysR family transcriptional regulator PA5437 compare
Pf6N2E2_5666 -2.3 -9.6 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_2514 -2.3 -4.6 regulator of length of O-antigen component of lipopolysaccharide chains compare
Pf6N2E2_3258 -2.3 -2.7 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_302 -2.3 -6.5 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_5571 -2.3 -3.3 hypothetical protein compare
Pf6N2E2_4372 -2.2 -5.1 FIG00460773: hypothetical protein compare
Pf6N2E2_2258 -2.2 -10.1 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_3170 -2.1 -6.8 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_4414 -2.1 -2.0 FIG00953808: hypothetical protein compare
Pf6N2E2_4638 -2.1 -3.4 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_5780 -2.1 -7.8 Periplasmic protease compare
Pf6N2E2_2586 -2.1 -1.9 Hpt domain protein compare
Pf6N2E2_3460 -2.0 -1.4 Exodeoxyribonuclease V beta chain (EC 3.1.11.5) compare
Pf6N2E2_2753 -2.0 -3.4 HtrA protease/chaperone protein compare
Pf6N2E2_3260 -1.9 -3.6 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_2938 -1.9 -4.0 Outer membrane lipoprotein compare
Pf6N2E2_1981 -1.9 -3.7 FIG051360: Periplasmic protein TonB, links inner and outer membranes compare
Pf6N2E2_3658 -1.8 -9.0 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_5667 -1.8 -7.0 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_3847 -1.8 -3.4 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_3198 -1.7 -1.1 tRNA-guanine transglycosylase (EC 2.4.2.29) compare
Pf6N2E2_2681 -1.6 -2.5 3-phosphoglycerate kinase compare
Pf6N2E2_3659 -1.6 -5.7 Protein fixF compare
Pf6N2E2_3373 -1.6 -5.6 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_2465 -1.6 -4.7 Cys regulon transcriptional activator CysB compare
Pf6N2E2_5520 -1.6 -6.3 Sensory box histidine kinase compare
Pf6N2E2_4360 -1.6 -7.7 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_2384 -1.5 -2.5 CmpX compare
Pf6N2E2_5573 -1.5 -5.1 Glycerol-3-phosphate regulon repressor, DeoR family compare
Pf6N2E2_4422 -1.5 -2.6 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_2746 -1.5 -5.2 Outer membrane protein NlpB, lipoprotein component of the protein assembly complex (forms a complex with YaeT, YfiO, and YfgL); Lipoprotein-34 precursor compare
Pf6N2E2_5579 -1.5 -5.7 Ornithine carbamoyltransferase (EC 2.1.3.3) compare
Pf6N2E2_5647 -1.5 -4.7 Transcriptional regulator, TetR family compare
Pf6N2E2_3663 -1.5 -7.7 Glycosyl transferase compare
Pf6N2E2_5711 -1.5 -5.6 tRNA(Cytosine32)-2-thiocytidine synthetase compare
Pf6N2E2_5705 -1.4 -3.4 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_3251 -1.4 -6.9 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_5728 -1.4 -3.2 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_4611 -1.4 -2.8 Ferric siderophore transport system, periplasmic binding protein TonB compare
Pf6N2E2_818 -1.4 -4.0 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_3487 -1.4 -8.2 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_3385 -1.4 -9.1 Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing compare
Pf6N2E2_3938 -1.4 -9.4 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_4612 -1.4 -4.2 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_3132 -1.4 -2.5 FIG00954548: hypothetical protein compare
Pf6N2E2_4059 -1.4 -1.5 DNA-binding protein HU-alpha compare
Pf6N2E2_3762 -1.4 -5.0 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_4801 -1.4 -10.9 yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
Pf6N2E2_2117 -1.4 -9.4 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) compare
Pf6N2E2_2286 -1.4 -3.4 FIG00953287: hypothetical protein compare
Pf6N2E2_3664 -1.3 -6.3 Carbamoyltransferase in large core OS assembly cluster compare
Pf6N2E2_2285 -1.3 -1.3 FIG00954079: hypothetical protein compare
Pf6N2E2_4049 -1.3 -7.0 Glycine cleavage system transcriptional activator compare
Pf6N2E2_3984 -1.3 -8.8 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_5153 -1.3 -4.4 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11) compare
Pf6N2E2_5950 -1.3 -3.6 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_2068 -1.3 -4.5 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) compare
Pf6N2E2_5258 -1.3 -6.4 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_5257 -1.3 -7.0 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_5425 -1.3 -1.8 hypothetical protein compare
Pf6N2E2_4799 -1.2 -9.2 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
Pf6N2E2_178 -1.2 -1.1 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_5678 -1.2 -4.2 hypothetical protein compare
Pf6N2E2_2707 -1.2 -2.9 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_1694 -1.2 -1.4 FMN reductase (EC 1.5.1.29) compare
Pf6N2E2_3257 -1.2 -1.9 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_2503 -1.2 -1.3 Glycosyl transferase, group 1 family protein compare
Pf6N2E2_4800 -1.2 -8.6 yeaH component of nitrogen-related signalling system yeaH (of yeaGH-ycgB) (from data) compare
Pf6N2E2_2189 -1.2 -4.6 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_2827 -1.2 -1.6 hypothetical protein compare
Pf6N2E2_3869 -1.2 -7.7 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_4988 -1.2 -3.1 Antiholin-like protein LrgA compare
Pf6N2E2_1932 -1.2 -3.0 Transcriptional regulator, IclR family compare
Pf6N2E2_2059 -1.2 -2.2 ChlI component of cobalt chelatase involved in B12 biosynthesis compare
Pf6N2E2_2072 -1.1 -2.0 hypothetical protein compare
Pf6N2E2_5160 -1.1 -0.9 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_2788 -1.1 -2.0 Transcriptional regulator, AsnC family compare
Pf6N2E2_5575 -1.1 -1.1 Glycerol uptake facilitator protein compare
Pf6N2E2_5906 -1.1 -2.8 Flp pilus assembly protein, pilin Flp compare
Pf6N2E2_4273 -1.1 -4.7 ATP-dependent DNA helicase UvrD/PcrA compare
Pf6N2E2_2869 -1.1 -1.3 Peptide deformylase (EC 3.5.1.88) compare
Pf6N2E2_4987 -1.1 -0.8 MaoC-like domain protein compare
Pf6N2E2_2506 -1.1 -8.0 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_2013 -1.1 -1.2 hypothetical protein compare
Pf6N2E2_3617 -1.1 -4.0 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) compare
Pf6N2E2_5527 -1.1 -1.6 Cold shock protein CspC compare
Pf6N2E2_58 -1.1 -2.3 tRNA-dihydrouridine synthase C (EC 1.-.-.-) compare
Pf6N2E2_3885 -1.1 -1.6 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7) compare
Pf6N2E2_3533 -1.1 -2.7 Cobalt-precorrin-8x methylmutase (EC 5.4.1.2) compare
Pf6N2E2_3569 -1.1 -1.4 hypothetical protein compare
Pf6N2E2_3344 -1.1 -2.6 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) AmpD compare
Pf6N2E2_70 -1.1 -2.7 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_895 -1.0 -3.0 Transcriptional regulator, HxlR family compare
Pf6N2E2_3833 -1.0 -3.7 hypothetical protein compare
Pf6N2E2_420 -1.0 -1.4 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_5086 -1.0 -1.1 hypothetical protein compare
Pf6N2E2_2756 -1.0 -3.6 RNA polymerase sigma-H factor AlgT compare
Pf6N2E2_5665 -1.0 -4.9 Succinylornithine transaminase (EC 2.6.1.81) compare
Pf6N2E2_4416 -1.0 -1.5 Tyrosine recombinase XerC compare
Pf6N2E2_5316 -1.0 -1.6 Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation compare
Pf6N2E2_3062 -1.0 -2.4 Membrane-associated zinc metalloprotease compare
Pf6N2E2_4678 -1.0 -1.0 hypothetical protein compare
Pf6N2E2_3199 -1.0 -2.6 S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) compare
Pf6N2E2_3300 -0.9 -2.0 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_4599 -0.9 -1.7 Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock) compare
Pf6N2E2_5676 -0.9 -3.7 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_4150 -0.9 -2.3 probable exported protein YPO0432 compare
Pf6N2E2_1994 -0.9 -3.4 putative lipoprotein compare
Pf6N2E2_3270 -0.9 -3.0 Hypothetical ATP-binding protein UPF0042, contains P-loop compare
Pf6N2E2_5338 -0.9 -7.7 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_5793 -0.9 -3.0 Aspartyl aminopeptidase compare
Pf6N2E2_4394 -0.9 -2.9 Putrescine utilization regulator compare
Pf6N2E2_5111 -0.9 -1.6 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_625 -0.9 -2.8 MoxR-like ATPase in aerotolerance operon compare
Pf6N2E2_2367 -0.9 -1.4 hypothetical protein compare
Pf6N2E2_2282 -0.9 -2.0 Predicted transcriptional regulator for fatty acid degradation FadQ, TetR family compare
Pf6N2E2_5703 -0.9 -1.9 outer membrane protein H1 compare
Pf6N2E2_1545 -0.9 -2.1 N-carbamoylputrescine amidase (EC 3.5.1.53) compare
Pf6N2E2_6082 -0.9 -2.3 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_50 -0.9 -4.2 Biosynthetic Aromatic amino acid aminotransferase alpha (EC 2.6.1.57) @ Aromatic-amino-acid aminotransferase (EC 2.6.1.57) compare
Pf6N2E2_5156 -0.9 -0.6 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_4680 -0.9 -2.5 HTH-type transcriptional regulator BetI compare
Pf6N2E2_5944 -0.9 -3.1 Ribosomal large subunit pseudouridine synthase C (EC 4.2.1.70) compare
Pf6N2E2_2442 -0.9 -5.9 sensor histidine kinase/response regulator compare
Pf6N2E2_2274 -0.9 -2.3 Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1) compare
Pf6N2E2_1753 -0.8 -2.8 FIG00643128: hypothetical protein compare
Pf6N2E2_3835 -0.8 -2.5 Carboxyl-terminal protease (EC 3.4.21.102) compare
Pf6N2E2_4989 -0.8 -2.1 LrgA-associated membrane protein LrgB compare
Pf6N2E2_2467 -0.8 -2.3 5'-nucleotidase (EC 3.1.3.5) compare
Pf6N2E2_5865 -0.8 -5.5 Chaperone protein HtpG compare


Specific Phenotypes

For 2 genes in this experiment

For stress Zinc sulfate heptahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Zinc sulfate heptahydrate across organisms