Experiment set11IT008 for Bacteroides thetaiotaomicron VPI-5482

Varel_Bryant_medium_Glucose with Anhydrotetracycline hydrochloride 0.0125 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Anhydrotetracycline hydrochloride (0.0125 mM) + Dimethyl Sulfoxide (0.125 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For stress Anhydrotetracycline hydrochloride in Bacteroides thetaiotaomicron VPI-5482

For stress Anhydrotetracycline hydrochloride across organisms

SEED Subsystems

Subsystem	#Specific
RNA processing and degradation, bacterial	1
Two cell division clusters relating to chromosome partitioning	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.