Experiment set11IT008 for Burkholderia phytofirmans PsJN
D-Glucose carbon source
Group: carbon sourceMedia: MOPS minimal media_noCarbon + D-Glucose (10 mM)
Culturing: BFirm_ML3_JBEI, tube, Aerobic, at 30 (C), shaken=200 rpm
Growth: about 2.4 generations
By: Allie Pearson on 8/26/19
Media components: 40 mM 3-(N-morpholino)propanesulfonic acid, 4 mM Tricine, 1.32 mM Potassium phosphate dibasic, 0.01 mM Iron (II) sulfate heptahydrate, 9.5 mM Ammonium chloride, 0.276 mM Aluminum potassium sulfate dodecahydrate, 0.0005 mM Calcium chloride, 0.525 mM Magnesium chloride hexahydrate, 50 mM Sodium Chloride, 3e-09 M Ammonium heptamolybdate tetrahydrate, 4e-07 M Boric Acid, 3e-08 M Cobalt chloride hexahydrate, 1e-08 M Copper (II) sulfate pentahydrate, 8e-08 M Manganese (II) chloride tetrahydrate, 1e-08 M Zinc sulfate heptahydrate
Specific Phenotypes
For 2 genes in this experiment
For carbon source D-Glucose in Burkholderia phytofirmans PsJN
For carbon source D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Experimental tye | 1 |
| Heme and Siroheme Biosynthesis | 1 |
| Purine conversions | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| adenosine nucleotides degradation III | 1 | 1 | 1 |
| heme b biosynthesis II (oxygen-independent) | 4 | 3 | 1 |
| superpathway of heme b biosynthesis from uroporphyrinogen-III | 6 | 4 | 1 |
| 3,8-divinyl-chlorophyllide a biosynthesis II (anaerobic) | 9 | 3 | 1 |
| superpathway of bacteriochlorophyll a biosynthesis | 26 | 6 | 1 |