Experiment set11IT007 for Bacteroides thetaiotaomicron VPI-5482

Compare to:

Varel_Bryant_medium_Glucose with Zinc Pyrithione 0.025 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Zinc Pyrithione (0.025 mM) + Dimethyl Sulfoxide (0.25 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 7 genes in this experiment

For stress Zinc Pyrithione in Bacteroides thetaiotaomicron VPI-5482

For stress Zinc Pyrithione across organisms

SEED Subsystems

Subsystem #Specific
Glycine and Serine Utilization 2
Carboxysome 1
Oxidative stress 1
Pyruvate Alanine Serine Interconversions 1
Respiratory dehydrogenases 1 1
Serine Biosynthesis 1
Transport of Zinc 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-serine degradation 3 3 3
L-tryptophan degradation II (via pyruvate) 3 3 2
L-cysteine degradation II 3 2 2
D-serine degradation 3 2 2
NADH to cytochrome bd oxidase electron transfer I 2 1 1
NADH to cytochrome bo oxidase electron transfer I 2 1 1
glycine betaine degradation III 7 4 3
felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis 5 2 2
glycine betaine degradation I 8 4 3
L-serine biosynthesis I 3 3 1
aerobic respiration III (alternative oxidase pathway) 3 2 1
glycine degradation 3 2 1
L-methionine biosynthesis II 6 3 2
superpathway of L-serine and glycine biosynthesis I 4 4 1
L-mimosine degradation 8 4 2
aerobic respiration I (cytochrome c) 4 2 1
glutathione-mediated detoxification I 8 2 2
NAD(P)/NADPH interconversion 6 2 1
Fe(II) oxidation 6 1 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 12 2
purine nucleobases degradation II (anaerobic) 24 12 3
superpathway of sulfate assimilation and cysteine biosynthesis 9 7 1