Experiment set11IT003 for Bacteroides thetaiotaomicron VPI-5482

Compare to:

Varel_Bryant_medium_Glucose with Trimethoprim 0.0125 mM

Group: stress
Media: Varel_Bryant_medium_Glucose + Trimethoprim (0.0125 mM) + Dimethyl Sulfoxide (0.125 vol%)
Culturing: Btheta_ML6a, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Hans on 6/18/18
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For stress Trimethoprim in Bacteroides thetaiotaomicron VPI-5482

For stress Trimethoprim across organisms

SEED Subsystems

Subsystem #Specific
Fermentations: Mixed acid 2
Butanol Biosynthesis 1
De Novo Purine Biosynthesis 1
Multidrug Resistance Efflux Pumps 1
Threonine anaerobic catabolism gene cluster 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
reductive monocarboxylic acid cycle 2 2 1
pyruvate fermentation to acetate IV 3 3 1
pyruvate fermentation to ethanol I 3 2 1
5-aminoimidazole ribonucleotide biosynthesis II 5 5 1
5-aminoimidazole ribonucleotide biosynthesis I 5 5 1
superpathway of 5-aminoimidazole ribonucleotide biosynthesis 6 6 1
L-threonine degradation I 6 5 1
superpathway of fermentation (Chlamydomonas reinhardtii) 9 7 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 10 1
mixed acid fermentation 16 12 1
superpathway of L-threonine metabolism 18 13 1
hexitol fermentation to lactate, formate, ethanol and acetate 19 14 1
superpathway of purine nucleotides de novo biosynthesis I 21 21 1
superpathway of N-acetylneuraminate degradation 22 16 1
superpathway of purine nucleotides de novo biosynthesis II 26 23 1
superpathway of histidine, purine, and pyrimidine biosynthesis 46 42 1