Experiment set11H43 for Pseudomonas stutzeri RCH2

LB with D-Cycloserine 0.032 mg/ml

Group: stress
Media: LB + D-Cycloserine (0.032 mg/ml)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 2/25/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 823 E5,E6

Specific Phenotypes

For 11 genes in this experiment

For stress D-Cycloserine in Pseudomonas stutzeri RCH2

For stress D-Cycloserine across organisms

SEED Subsystems

Subsystem	#Specific
Control of cell elongation - division cycle in Bacilli	1
DNA Repair Base Excision	1
Flavodoxin	1
Lysine Biosynthesis DAP Pathway	1
Na(+)-translocating NADH-quinone oxidoreductase and rnf-like group of electron transport complexes	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1
Purine conversions	1
Ribosomal protein S12p Asp methylthiotransferase	1
tRNA processing	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Puromycin biosynthesis
• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• Tryptophan metabolism
• Phenylalanine, tyrosine and tryptophan biosynthesis
• Lipopolysaccharide biosynthesis
• Methane metabolism
• Drug metabolism - other enzymes
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superoxide radicals degradation	2	2	1
guanine and guanosine salvage II	2	1	1
methanol oxidation to formaldehyde IV	2	1	1
guanine and guanosine salvage I	2	1	1
xanthine and xanthosine salvage	2	1	1
L-tryptophan biosynthesis	6	6	2
ethanol degradation IV	3	3	1
adenine salvage	3	2	1
superpathway of guanine and guanosine salvage	3	1	1
reactive oxygen species degradation	4	4	1
adenine and adenosine salvage III	4	2	1
DIBOA-glucoside biosynthesis	6	1	1
superpathway of L-tryptophan biosynthesis	13	13	2
superpathway of aromatic amino acid biosynthesis	18	18	2
L-lysine biosynthesis I	9	9	1
superpathway of benzoxazinoid glucosides biosynthesis	13	1	1
superpathway of purine nucleotide salvage	14	11	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	16	1
aspartate superpathway	25	22	1
superpathway of chorismate metabolism	59	44	2