Experiment set11H27 for Pseudomonas stutzeri RCH2

LB with Bacitracin 0.25 mg/ml

Group: stress
Media: LB + Bacitracin (0.25 mg/ml)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 2/25/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 823 B3,B4

Specific Phenotypes

For 37 genes in this experiment

For stress Bacitracin in Pseudomonas stutzeri RCH2

For stress Bacitracin across organisms

SEED Subsystems

Subsystem	#Specific
Flagellum	4
Flagellar motility	2
Oxidative stress	2
Alanine biosynthesis	1
Branched-Chain Amino Acid Biosynthesis	1
Isoleucine degradation	1
Leucine Biosynthesis	1
Leucine Degradation and HMG-CoA Metabolism	1
Multidrug Resistance Efflux Pumps	1
Purine conversions	1
Pyruvate Alanine Serine Interconversions	1
Valine degradation	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Ascorbate and aldarate metabolism
• Ubiquinone and menaquinone biosynthesis
• Valine, leucine and isoleucine degradation
• Valine, leucine and isoleucine biosynthesis
• Glutathione metabolism
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• Lipopolysaccharide biosynthesis
• Pantothenate and CoA biosynthesis
• Folate biosynthesis
• Drug metabolism - other enzymes
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from histidine and purine

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-alanine biosynthesis I	2	2	1
(1,4)-β-D-xylan degradation	2	1	1
guanine and guanosine salvage II	2	1	1
guanine and guanosine salvage I	2	1	1
xanthine and xanthosine salvage	2	1	1
L-isoleucine biosynthesis V	3	3	1
adenine salvage	3	2	1
L-valine degradation III (oxidative Stickland reaction)	3	2	1
L-valine degradation II	3	2	1
L-isoleucine degradation II	3	2	1
L-leucine degradation V (oxidative Stickland reaction)	3	2	1
cellulose and hemicellulose degradation (cellulolosome)	3	2	1
L-isoleucine degradation III (oxidative Stickland reaction)	3	2	1
L-leucine degradation III	3	2	1
superpathway of guanine and guanosine salvage	3	1	1
superpathway of L-alanine biosynthesis	4	4	1
L-valine biosynthesis	4	4	1
adenine and adenosine salvage III	4	2	1
L-leucine degradation IV (reductive Stickland reaction)	5	1	1
superpathway of branched chain amino acid biosynthesis	17	17	3
L-leucine biosynthesis	6	6	1
L-isoleucine biosynthesis IV	6	5	1
L-isoleucine degradation I	6	5	1
L-leucine degradation I	6	5	1
L-isoleucine biosynthesis I (from threonine)	7	7	1
L-isoleucine biosynthesis III	7	4	1
L-valine degradation I	8	6	1
L-isoleucine biosynthesis II	8	4	1
superpathway of L-isoleucine biosynthesis I	13	13	1
superpathway of purine nucleotide salvage	14	11	1
superpathway of L-threonine metabolism	18	13	1
odd iso-branched-chain fatty acid biosynthesis	34	24	1
even iso-branched-chain fatty acid biosynthesis	34	24	1
anteiso-branched-chain fatty acid biosynthesis	34	24	1