Experiment set11H26 for Pseudomonas stutzeri RCH2

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LB with Phosphomycin disodium salt 0.012 mg/ml

Group: stress
Media: LB + Phosphomycin disodium salt (0.012 mg/ml)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 2/25/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 824 A7,A8

Specific Phenotypes

For 15 genes in this experiment

For stress Phosphomycin disodium salt in Pseudomonas stutzeri RCH2

For stress Phosphomycin disodium salt across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 3
ATP-dependent RNA helicases, bacterial 1
Alanine biosynthesis 1
Alkylphosphonate utilization 1
Branched-Chain Amino Acid Biosynthesis 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Isoleucine degradation 1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway 1
Lactose and Galactose Uptake and Utilization 1
Leucine Biosynthesis 1
Leucine Degradation and HMG-CoA Metabolism 1
N-linked Glycosylation in Bacteria 1
NAD and NADP cofactor biosynthesis global 1
Nudix proteins (nucleoside triphosphate hydrolases) 1
Pyruvate Alanine Serine Interconversions 1
Rhamnose containing glycans 1
Threonine and Homoserine Biosynthesis 1
Valine degradation 1
linker unit-arabinogalactan synthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-galactose biosynthesis 1 1 1
L-aspartate biosynthesis 1 1 1
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
UDP-N-acetyl-D-galactosamine biosynthesis I 1 1 1
L-aspartate degradation I 1 1 1
L-glutamate degradation II 2 2 1
L-alanine biosynthesis I 2 2 1
UDP-N-acetyl-α-D-quinovosamine biosynthesis 2 2 1
UDP-α-D-galactofuranose biosynthesis 2 2 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
malate/L-aspartate shuttle pathway 2 1 1
atromentin biosynthesis 2 1 1
L-tyrosine degradation II 2 1 1
L-isoleucine biosynthesis V 3 3 1
L-tyrosine biosynthesis I 3 3 1
L-phenylalanine biosynthesis I 3 3 1
L-isoleucine degradation II 3 2 1
L-asparagine degradation III (mammalian) 3 2 1
L-valine degradation III (oxidative Stickland reaction) 3 2 1
L-leucine degradation V (oxidative Stickland reaction) 3 2 1
L-phenylalanine degradation II (anaerobic) 3 2 1
prenylated FMNH2 biosynthesis 3 2 1
L-valine degradation II 3 2 1
L-isoleucine degradation III (oxidative Stickland reaction) 3 2 1
L-leucine degradation III 3 2 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
sulfolactate degradation III 3 1 1
D-galactose detoxification 3 1 1
(R)-cysteate degradation 3 1 1
superpathway of L-alanine biosynthesis 4 4 1
L-valine biosynthesis 4 4 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 3 1
L-phenylalanine degradation III 4 2 1
L-tyrosine degradation III 4 2 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
L-tyrosine degradation I 5 5 1
trans-4-hydroxy-L-proline degradation I 5 3 1
D-galactose degradation I (Leloir pathway) 5 2 1
superpathway of plastoquinol biosynthesis 5 2 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
L-leucine degradation IV (reductive Stickland reaction) 5 1 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 2
superpathway of branched chain amino acid biosynthesis 17 17 3
L-leucine biosynthesis 6 6 1
superpathway of L-threonine biosynthesis 6 6 1
TCA cycle VIII (Chlamydia) 6 5 1
L-leucine degradation I 6 5 1
L-isoleucine degradation I 6 5 1
L-isoleucine biosynthesis IV 6 5 1
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
superpathway of sulfolactate degradation 6 2 1
coenzyme M biosynthesis II 6 1 1
superpathway of L-isoleucine biosynthesis I 13 13 2
L-isoleucine biosynthesis I (from threonine) 7 7 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 2
L-isoleucine biosynthesis III 7 4 1
UDP-N-acetyl-D-galactosamine biosynthesis II 7 4 1
anaerobic energy metabolism (invertebrates, cytosol) 7 4 1
stachyose degradation 7 2 1
L-valine degradation I 8 6 1
L-isoleucine biosynthesis II 8 4 1
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 3 1
UDP-sugars interconversion 9 1 1
teichuronic acid biosynthesis (B. subtilis 168) 9 1 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
rosmarinic acid biosynthesis I 10 1 1
colanic acid building blocks biosynthesis 11 10 1
(S)-reticuline biosynthesis I 11 1 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
indole-3-acetate biosynthesis II 12 3 1
superpathway of rosmarinic acid biosynthesis 14 1 1
superpathway of anaerobic energy metabolism (invertebrates) 17 9 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
superpathway of L-threonine metabolism 18 13 1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 18 2 1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis 24 9 1
aspartate superpathway 25 22 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
superpathway of chorismate metabolism 59 44 2
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 14 1
anteiso-branched-chain fatty acid biosynthesis 34 24 1
even iso-branched-chain fatty acid biosynthesis 34 24 1
odd iso-branched-chain fatty acid biosynthesis 34 24 1
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1