Experiment set11H25 for Pseudomonas stutzeri RCH2

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LB with Phosphomycin disodium salt 0.012 mg/ml

Group: stress
Media: LB + Phosphomycin disodium salt (0.012 mg/ml)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 2/25/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 823 A7,A8

Specific Phenotypes

For 13 genes in this experiment

For stress Phosphomycin disodium salt in Pseudomonas stutzeri RCH2

For stress Phosphomycin disodium salt across organisms

SEED Subsystems

Subsystem #Specific
ABC transporter alkylphosphonate (TC 3.A.1.9.1) 3
Alkylphosphonate utilization 2
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
NAD and NADP cofactor biosynthesis global 1
Nudix proteins (nucleoside triphosphate hydrolases) 1
Threonine and Homoserine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
3-(4-hydroxyphenyl)pyruvate biosynthesis 1 1 1
L-aspartate biosynthesis 1 1 1
L-aspartate degradation I 1 1 1
L-glutamate degradation II 2 2 1
malate/L-aspartate shuttle pathway 2 1 1
L-tryptophan degradation IV (via indole-3-lactate) 2 1 1
atromentin biosynthesis 2 1 1
L-tyrosine degradation II 2 1 1
L-phenylalanine biosynthesis I 3 3 1
L-tyrosine biosynthesis I 3 3 1
L-phenylalanine degradation II (anaerobic) 3 2 1
prenylated FMNH2 biosynthesis 3 2 1
L-asparagine degradation III (mammalian) 3 2 1
(R)-cysteate degradation 3 1 1
indole-3-acetate biosynthesis VI (bacteria) 3 1 1
sulfolactate degradation III 3 1 1
L-tyrosine degradation IV (to 4-methylphenol) 3 1 1
superpathway of L-aspartate and L-asparagine biosynthesis 4 3 1
L-tyrosine degradation III 4 2 1
L-phenylalanine degradation III 4 2 1
L-tryptophan degradation VIII (to tryptophol) 4 1 1
L-tyrosine degradation I 5 5 1
trans-4-hydroxy-L-proline degradation I 5 3 1
superpathway of plastoquinol biosynthesis 5 2 1
L-tryptophan degradation XIII (reductive Stickland reaction) 5 1 1
4-hydroxybenzoate biosynthesis I (eukaryotes) 5 1 1
L-phenylalanine degradation VI (reductive Stickland reaction) 5 1 1
L-tyrosine degradation V (reductive Stickland reaction) 5 1 1
C4 photosynthetic carbon assimilation cycle, NAD-ME type 11 6 2
superpathway of L-threonine biosynthesis 6 6 1
TCA cycle VIII (Chlamydia) 6 5 1
superpathway of sulfolactate degradation 6 2 1
methylgallate degradation 6 2 1
coenzyme M biosynthesis II 6 1 1
C4 photosynthetic carbon assimilation cycle, PEPCK type 14 9 2
anaerobic energy metabolism (invertebrates, cytosol) 7 4 1
protocatechuate degradation I (meta-cleavage pathway) 8 3 1
superpathway of aromatic amino acid biosynthesis 18 18 2
superpathway of L-methionine biosynthesis (transsulfuration) 9 7 1
L-phenylalanine degradation IV (mammalian, via side chain) 9 3 1
superpathway of L-phenylalanine biosynthesis 10 10 1
superpathway of L-tyrosine biosynthesis 10 10 1
superpathway of vanillin and vanillate degradation 10 3 1
rosmarinic acid biosynthesis I 10 1 1
(S)-reticuline biosynthesis I 11 1 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 12 1
indole-3-acetate biosynthesis II 12 3 1
syringate degradation 12 3 1
phosalacine biosynthesis 25 4 2
phosphinothricin tripeptide biosynthesis 25 4 2
superpathway of L-isoleucine biosynthesis I 13 13 1
superpathway of rosmarinic acid biosynthesis 14 1 1
superpathway of anaerobic energy metabolism (invertebrates) 17 9 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 16 1
aspartate superpathway 25 22 1
anaerobic aromatic compound degradation (Thauera aromatica) 27 3 1
superpathway of chorismate metabolism 59 44 2
Methanobacterium thermoautotrophicum biosynthetic metabolism 56 21 1