Experiment set11H12 for Pseudomonas stutzeri RCH2

LB with Sodium nitrate 200 mM

Group: stress
Media: LB + Sodium nitrate (200 mM)
Culturing: psRCH2_ML7c, 48 well microplate; Tecan Infinite F200, Aerobic, at 30 (C), shaken=orbital
By: Kelly on 2/24/2014
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: 822 E3,E4

Specific Phenotypes

For 8 genes in this experiment

For stress Sodium nitrate in Pseudomonas stutzeri RCH2

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem	#Specific
Central meta-cleavage pathway of aromatic compound degradation	1
Choline and Betaine Uptake and Betaine Biosynthesis	1
NAD and NADP cofactor biosynthesis global	1
NAD regulation	1
Oxidative stress	1
Photorespiration (oxidative C2 cycle)	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism
• Tryptophan metabolism
• Nucleotide sugars metabolism
• Methane metabolism
• Nicotinate and nicotinamide metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
superoxide radicals degradation	2	2	1
methanol oxidation to formaldehyde IV	2	1	1
ethanol degradation IV	3	3	1
CDP-4-dehydro-3,6-dideoxy-D-glucose biosynthesis	3	1	1
reactive oxygen species degradation	4	4	1
NAD de novo biosynthesis IV (anaerobic)	6	5	1
NAD de novo biosynthesis I	6	5	1
superpathway of CDP-glucose-derived O-antigen building blocks biosynthesis	8	1	1
nicotine biosynthesis	9	3	1
superpathway of nicotine biosynthesis	12	4	1
aspartate superpathway	25	22	1