Experiment set10S424 for Enterobacter sp. TBS_079
Cytosine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + Cytosine (2.5 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 12 genes in this experiment
For nitrogen source Cytosine in Enterobacter sp. TBS_079
For nitrogen source Cytosine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Pyrimidine utilization | 5 |
| Colanic acid biosynthesis | 4 |
| De Novo Pyrimidine Synthesis | 1 |
| Urea decomposition | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Tryptophan metabolism
- Sphingolipid metabolism
- Ascorbate and aldarate metabolism
- Biosynthesis of steroids
- Ubiquinone and menaquinone biosynthesis
- Pyrimidine metabolism
- Arginine and proline metabolism
- gamma-Hexachlorocyclohexane degradation
- Benzoxazinone biosynthesis
- Aminophosphonate metabolism
- High-mannose type N-glycan biosynthesis
- Nucleotide sugars metabolism
- Glycerophospholipid metabolism
- Naphthalene and anthracene degradation
- 1,4-Dichlorobenzene degradation
- Methane metabolism
- Limonene and pinene degradation
- Diterpenoid biosynthesis
- Brassinosteroid biosynthesis
- Carotenoid biosynthesis - General
- Phenylpropanoid biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of terpenoids and steroids
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pyrimidine ribonucleosides salvage III | 2 | 2 | 1 |
| pyrimidine nucleobases salvage II | 2 | 2 | 1 |
| colanic acid (Escherichia coli K12) biosynthesis | 11 | 10 | 3 |
| uracil degradation III | 5 | 5 | 1 |
| dibenzothiophene desulfurization | 5 | 1 | 1 |
| bacterial bioluminescence | 8 | 4 | 1 |
| Streptococcus pneumoniae serotype 2 capsular polysaccharide biosynthesis | 8 | 1 | 1 |
| xanthan biosynthesis | 10 | 1 | 1 |
| acetan biosynthesis | 11 | 1 | 1 |