Experiment set10S422 for Enterobacter sp. TBS_079
Cytidine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + Cytidine (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 2 genes in this experiment
For nitrogen source Cytidine in Enterobacter sp. TBS_079
For nitrogen source Cytidine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| NAD and NADP cofactor biosynthesis global | 1 |
| NAD regulation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Nicotinate and nicotinamide metabolism
- Purine metabolism
- Pyrimidine metabolism
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 3 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 9 | 3 |
| inosine 5'-phosphate degradation | 4 | 3 | 1 |
| guanosine nucleotides degradation II | 4 | 3 | 1 |
| guanosine nucleotides degradation III | 4 | 3 | 1 |
| purine nucleotides degradation I (plants) | 12 | 8 | 3 |
| adenosine nucleotides degradation I | 8 | 5 | 2 |
| guanosine nucleotides degradation I | 4 | 2 | 1 |
| adenosine nucleotides degradation II | 5 | 3 | 1 |
| NAD de novo biosynthesis IV (anaerobic) | 6 | 5 | 1 |
| NAD de novo biosynthesis I | 6 | 5 | 1 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 4 | 1 |
| superpathway of purines degradation in plants | 18 | 11 | 3 |
| ureide biosynthesis | 7 | 6 | 1 |
| nicotine biosynthesis | 9 | 3 | 1 |
| tunicamycin biosynthesis | 9 | 2 | 1 |
| NAD salvage (plants) | 11 | 5 | 1 |
| superpathway of nicotine biosynthesis | 12 | 4 | 1 |
| aspartate superpathway | 25 | 24 | 1 |