Experiment set10S418 for Enterobacter sp. TBS_079

Adenine hydrochloride hydrate nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + Adenine hydrochloride hydrate (2.5 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 16 genes in this experiment

For nitrogen source Adenine hydrochloride hydrate in Enterobacter sp. TBS_079

For nitrogen source Adenine hydrochloride hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Deoxyribose and Deoxynucleoside Catabolism	2
Purine conversions	2
Adenosyl nucleosidases	1
Calvin-Benson cycle	1
Colanic acid biosynthesis	1
DNA-binding regulatory proteins, strays	1
De Novo Pyrimidine Synthesis	1
Multidrug Resistance Efflux Pumps	1
Pentose phosphate pathway	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Pyrimidine metabolism
• Pentose phosphate pathway
• Nicotinate and nicotinamide metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Alanine and aspartate metabolism
• Carbon fixation in photosynthetic organisms
• Biosynthesis of ansamycins
• Biosynthesis of phenylpropanoids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
purine ribonucleosides degradation	6	6	6
purine deoxyribonucleosides degradation I	4	4	4
purine deoxyribonucleosides degradation II	3	3	3
adenine and adenosine salvage III	4	4	3
superpathway of purine deoxyribonucleosides degradation	7	7	5
pentose phosphate pathway (partial)	3	3	2
adenine and adenosine salvage V	3	3	2
purine nucleotides degradation II (aerobic)	11	9	7
adenosine nucleotides degradation II	5	3	3
guanine and guanosine salvage I	2	2	1
adenine and adenosine salvage I	2	2	1
xanthine and xanthosine salvage	2	2	1
guanosine nucleotides degradation III	4	3	2
inosine 5'-phosphate degradation	4	3	2
pentose phosphate pathway (non-oxidative branch) I	5	5	2
superpathway of pyrimidine deoxyribonucleosides degradation	6	6	2
superpathway of guanine and guanosine salvage	3	3	1
NAD salvage pathway III (to nicotinamide riboside)	3	3	1
pyrimidine deoxyribonucleosides degradation	3	3	1
2-deoxy-α-D-ribose 1-phosphate degradation	3	3	1
pentose phosphate pathway (non-oxidative branch) II	6	5	2
ureide biosynthesis	7	6	2
UTP and CTP dephosphorylation I	7	5	2
pentose phosphate pathway	8	8	2
guanosine nucleotides degradation II	4	3	1
purine nucleotides degradation I (plants)	12	8	3
adenosine nucleotides degradation I	8	5	2
guanosine nucleotides degradation I	4	2	1
formaldehyde assimilation II (assimilatory RuMP Cycle)	9	7	2
Rubisco shunt	10	9	2
nucleoside and nucleotide degradation (archaea)	10	4	2
UMP biosynthesis I	6	6	1
UMP biosynthesis II	6	6	1
formaldehyde assimilation III (dihydroxyacetone cycle)	12	11	2
UMP biosynthesis III	6	5	1
superpathway of guanosine nucleotides degradation (plants)	6	4	1
superpathway of purines degradation in plants	18	11	3
fluoroacetate and fluorothreonine biosynthesis	6	1	1
nucleoside and nucleotide degradation (halobacteria)	6	1	1
Calvin-Benson-Bassham cycle	13	10	2
superpathway of purine nucleotide salvage	14	13	2
superpathway of glucose and xylose degradation	17	16	2
oxygenic photosynthesis	17	11	2
superpathway of pyrimidine ribonucleotides de novo biosynthesis	9	9	1
tunicamycin biosynthesis	9	2	1
colanic acid (Escherichia coli K12) biosynthesis	11	10	1
NAD salvage (plants)	11	5	1
ethene biosynthesis V (engineered)	25	18	2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered)	26	19	2
1-butanol autotrophic biosynthesis (engineered)	27	19	2
Bifidobacterium shunt	15	12	1
salinosporamide A biosynthesis	15	3	1
arsenic detoxification (mammals)	17	8	1
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis	18	18	1
superpathway of histidine, purine, and pyrimidine biosynthesis	46	46	1