Experiment set10S414 for Enterobacter sp. TBS_079
N-Acetyl-D-Glucosamine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + N-Acetyl-D-Glucosamine (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 7 genes in this experiment
For nitrogen source N-Acetyl-D-Glucosamine in Enterobacter sp. TBS_079
For nitrogen source N-Acetyl-D-Glucosamine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Chitin and N-acetylglucosamine utilization | 2 |
| Sialic Acid Metabolism | 2 |
| Type 4 secretion and conjugative transfer | 1 |
| UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Glycolysis / Gluconeogenesis
- Nucleotide sugars metabolism
- Aminosugars metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Ascorbate and aldarate metabolism
- Starch and sucrose metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| N-acetylglucosamine degradation I | 2 | 2 | 1 |
| N-acetylglucosamine degradation II | 3 | 3 | 1 |
| superpathway of N-acetylglucosamine, N-acetylmannosamine and N-acetylneuraminate degradation | 6 | 3 | 1 |
| UDP-N-acetyl-D-galactosamine biosynthesis II | 7 | 5 | 1 |
| chitin degradation I (archaea) | 7 | 2 | 1 |
| chitin derivatives degradation | 8 | 4 | 1 |
| superpathway of N-acetylneuraminate degradation | 22 | 19 | 1 |