Experiment set10S409 for Enterobacter sp. TBS_079

L-Citrulline nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + L-Citrulline (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 5 genes in this experiment

For nitrogen source L-Citrulline in Enterobacter sp. TBS_079

For nitrogen source L-Citrulline across organisms

SEED Subsystems

Subsystem	#Specific
Arginine Deiminase Pathway	2
Arginine and Ornithine Degradation	2
Arginine Biosynthesis extended	1
Colanic acid biosynthesis	1
Hydantoin metabolism	1
MLST	1
Multidrug Resistance Efflux Pumps	1
Polyamine Metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Purine metabolism
• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups
• Glutamate metabolism
• Nitrogen metabolism
• T cell receptor signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-citrulline degradation	3	3	2
L-arginine degradation V (arginine deiminase pathway)	4	4	2
allantoin degradation to ureidoglycolate II (ammonia producing)	4	1	1
allantoin degradation IV (anaerobic)	9	3	2
L-arginine degradation XIII (reductive Stickland reaction)	5	4	1
urea cycle	5	3	1
allantoin degradation to glyoxylate III	5	1	1
allantoin degradation to glyoxylate II	5	1	1
L-arginine degradation XIV (oxidative Stickland reaction)	6	2	1
L-citrulline biosynthesis	8	6	1
superpathway of allantoin degradation in plants	8	2	1
L-arginine biosynthesis I (via L-ornithine)	9	9	1
L-arginine biosynthesis IV (archaea)	9	4	1
L-arginine biosynthesis II (acetyl cycle)	10	9	1
superpathway of L-citrulline metabolism	12	8	1
guadinomine B biosynthesis	13	2	1
superpathway of arginine and polyamine biosynthesis	17	16	1