Experiment set10S407 for Enterobacter sp. TBS_079

Gly-DL-Asp nitrogen source

Group: nitrogen source
Media: RCH2_defined_Glucose_noNitrogen + Gly-DL-Asp (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 10 genes in this experiment

For nitrogen source Gly-DL-Asp in Enterobacter sp. TBS_079

For nitrogen source Gly-DL-Asp across organisms

SEED Subsystems

Subsystem	#Specific
ABC transporter dipeptide (TC 3.A.1.5.2)	5
Glycine and Serine Utilization	2
Glycine cleavage system	2
Photorespiration (oxidative C2 cycle)	2
Bacterial Chemotaxis	1
DNA-binding regulatory proteins, strays	1
Type 4 secretion and conjugative transfer	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• One carbon pool by folate
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glycine biosynthesis II	3	3	2
glycine cleavage	3	3	2
glycine degradation	3	3	1
folate transformations III (E. coli)	9	9	1
reductive glycine pathway of autotrophic CO2 fixation	9	6	1
photorespiration I	9	5	1
photorespiration III	9	5	1
photorespiration II	10	6	1
folate transformations II (plants)	11	10	1
folate transformations I	13	9	1