Experiment set10S397 for Enterobacter sp. TBS_079
L-Alanine nitrogen source
Group: nitrogen sourceMedia: RCH2_defined_Glucose_noNitrogen + L-Alanine (10 mM)
Culturing: Enterobacter_TBS_079_ML3, microplate, Aerobic, at 30 (C)
By: Robin on 6/19/24
Media components: 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Specific Phenotypes
For 6 genes in this experiment
For nitrogen source L-Alanine in Enterobacter sp. TBS_079
For nitrogen source L-Alanine across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Pyruvate Alanine Serine Interconversions | 2 |
| Alanine biosynthesis | 1 |
| Glycine cleavage system | 1 |
| Orphan regulatory proteins | 1 |
| Respiratory dehydrogenases 1 | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Purine metabolism
- Pyrimidine metabolism
- Alanine and aspartate metabolism
- Phenylalanine metabolism
- D-Alanine metabolism
- Nicotinate and nicotinamide metabolism
- Nitrogen metabolism
- Biosynthesis of alkaloids derived from histidine and purine
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| L-alanine degradation I | 2 | 2 | 2 |
| alanine racemization | 1 | 1 | 1 |
| NAD salvage pathway III (to nicotinamide riboside) | 3 | 3 | 1 |
| UTP and CTP dephosphorylation I | 7 | 5 | 2 |
| purine nucleotides degradation II (aerobic) | 11 | 9 | 3 |
| guanosine nucleotides degradation III | 4 | 3 | 1 |
| guanosine nucleotides degradation II | 4 | 3 | 1 |
| inosine 5'-phosphate degradation | 4 | 3 | 1 |
| purine nucleotides degradation I (plants) | 12 | 8 | 3 |
| adenosine nucleotides degradation I | 8 | 5 | 2 |
| guanosine nucleotides degradation I | 4 | 2 | 1 |
| adenosine nucleotides degradation II | 5 | 3 | 1 |
| superpathway of guanosine nucleotides degradation (plants) | 6 | 4 | 1 |
| superpathway of purines degradation in plants | 18 | 11 | 3 |
| ureide biosynthesis | 7 | 6 | 1 |
| anaerobic energy metabolism (invertebrates, cytosol) | 7 | 5 | 1 |
| tunicamycin biosynthesis | 9 | 2 | 1 |
| ansatrienin biosynthesis | 9 | 1 | 1 |
| NAD salvage (plants) | 11 | 5 | 1 |
| cyclosporin A biosynthesis | 15 | 2 | 1 |
| superpathway of anaerobic energy metabolism (invertebrates) | 17 | 10 | 1 |